山楂醇提物对果蝇抗衰老的研究

发布时间：2018-05-13 10:31

本文选题：山楂 + 果蝇　；参考：《天津科技大学》2011年硕士论文

【摘要】：山楂为蔷薇科落叶灌木或小乔木,全国各地均有栽培,为药食同源植物。山楂果中含有黄酮类、熊果酸、皂苷、糖类、有机酸类、色素等多种活性成分。这些化合物在心血管的治疗与保健方面以及抗氧化等方面越来越受到人们的关注。果蝇是较为普遍的研究抗衰老模型之一,可用于研究衰老对基因的影响与作用。已有研究证明,果蝇的衰老基因与人类的衰老基因高度相似。目前,果蝇已被广泛应用于国内外的抗衰老研究实验中,但是应用于山楂的抗衰老研究鲜见报道。本文以山楂醇提物为原料,以果蝇为动物模型,对山楂的抗衰老作用进行评价,探索研究山楂醇提物的体内抗氧化性。旨在进一步研究探索山楂的体内抗氧化、抗衰老作用,为山楂抗衰老作用的研究与开发提供理论依据。第一,检测山楂提取物中抗氧化物质的种类与含量。以芦丁为标准品,通过紫外分光光度法,测得总黄酮含量为58.60%：经高效液相色谱法,测得原花青素B2、表儿茶素、金丝桃苷三种物质的含量分别为12.72%、7.34%、3.31%。第二,进行体内抗氧化活性的研究。建立正常果蝇模型,分别饲喂不同剂量的山楂醇提物。各组相比较,饲喂山楂醇提物的果蝇较空白组寿命延长；总SOD、 CuZn-SOD、CAT酶活力均显著提高(P0.01),且在一定范围内随剂量增大活力增强,超出此范围,上述作用降低；在一定浓度范围内MDA含量降低,各组之间呈现量效关系,浓度超过此范围,作用减弱；不同剂量组之间也呈现差异性显著。建立高脂模型。饲喂不同剂量的山楂醇提物,随剂量的增大寿命延长；总SOD、 CuZn-SOD、CAT酶活力均显著提高(P0.01),且在一定范围内随剂量增大活力增强,超出此范围,作用降低；在一定浓度范围内MDA含量降低,各组之间呈现量效关系,浓度超过此范围,作用减弱；不同剂量组之间也呈现差异性显著。建立急性致死模型。分别用双氧水、Paraquat作用于已饲喂不同剂量山楂醇提物的果蝇,诱导其体内的羟自由基与超氧阴离子氧化,经寿命实验验证,高剂量组的果蝇寿命高于低剂量组。经上述实验证明,山楂提取物具有较强的体内抗氧化性,可开发抗衰老类保健食品。
[Abstract]:Hawthorn is a deciduous bush or small tree of Rosaceae. Hawthorn fruit contains flavonoids, ursolic acid, saponins, carbohydrates, organic acids, pigments and other active ingredients. More and more attention has been paid to these compounds in cardiovascular treatment and health care as well as antioxidant. Drosophila is one of the most popular anti-aging models, which can be used to study the effect of senescence on genes. Studies have shown that the aging genes of Drosophila are highly similar to those of humans. At present, Drosophila has been widely used in anti-aging experiments at home and abroad, but the anti-aging research on Hawthorn is rarely reported. In this paper, the anti-aging effect of Hawthorn was evaluated by using Hawthorn alcohol extract as raw material and Drosophila as animal model, and the antioxidation of Hawthorn alcohol extract in vivo was studied. The aim of this study was to explore the antioxidation and anti-aging effects of Hawthorn in vivo and to provide theoretical basis for the research and development of anti-aging effect of Hawthorn. First, the kinds and contents of antioxidants in Hawthorn extracts were detected. The content of total flavonoids was 58.60 by UV spectrophotometry, and the contents of proanthocyanidin B _ 2, epigallocatechin and hyperoside were 12.727.34cm and 3.31 respectively by HPLC. Second, the study of antioxidant activity in vivo. A normal Drosophila model was established and fed with different doses of Hawthorn alcohol extract. Compared with the control group, Drosophila melanogaster fed with Hawthorn alcohol extract had longer life span, total SOD, CuZn-SOD cat enzyme activity increased significantly, and the activity increased with the increase of dosage, beyond this range, the above effects decreased. In a certain concentration range, the content of MDA decreased, there was a dose-effect relationship between the groups, the concentration exceeded this range, the effect was weakened, and there were significant differences among different dose groups. Establish high fat model. The activity of total SOD and CuZn-SODX cat increased significantly with the increase of dose, and the activity of total SOD and CuZn-SODX cat increased with the increase of dose, and the activity increased with the increase of dose, and the effect decreased. In a certain concentration range, the content of MDA decreased, there was a dose-effect relationship between the groups, the concentration exceeded this range, the effect was weakened, and there were significant differences among different dose groups. Acute death model was established. Drosophila melanogaster fed with different doses of Hawthorn alcohol extract was treated with Paraquat hydrogen peroxide to induce hydroxyl radical and superoxide anion oxidation. The life span of fruit fly in high dose group was higher than that in low dose group. The above experiments proved that Hawthorn extract has strong antioxidation in vivo and can be used to develop anti-aging health food.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R285.5;R-332

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