中国恒河猴SIV急性感染期模型CD4细胞和MBL的变化

发布时间：2018-06-03 12:59

本文选题：甘露糖结合凝集素 + 淋巴结细胞　；参考：《中国艾滋病性病》2017年03期

【摘要】：目的研究中国恒河猴猴免疫缺陷病毒(SIV)mac239急性期感染模型外,周血CD4+T淋巴细胞(简称CD4细胞)、淋巴结CD4细胞和血清甘露糖结合凝集素(MBL)的变化情况,探索感染早期固有免疫与适应性免疫的病理变化。方法选用5只健康中国恒河猴,静脉接种SIVmac239病毒株,分别于感染前、感染后2周、4周和10周检测血常规、血浆病毒载量,流式细胞仪分析外周血淋巴细胞亚群,免疫组织化学染色法观察淋巴结CD4细胞,酶联免疫吸附试验法检测血清MBL。结果感染后CD4细胞比值较感染前降低27.3%(P0.05),CD8细胞比值较感染前升高44.3%(P0.05),外周血CD4细胞绝对数下降23.6%,淋巴结CD4细胞上升14.9%,但是差异无统计学意义。血清MBL在感染后10周内持续下降至感染前水平的24.5%(P0.01)。结论 SIV急性感染期固有免疫破坏显著,适应性免疫主要表现为体液免疫与细胞免疫的失衡。
[Abstract]:Objective to study the changes of peripheral blood CD4 T lymphocytes (CD4 cells, CD4 cells and serum mannose binding lectin) in Chinese rhesus monkey (Rhesus monkey) in acute stage infection model. To explore the pathological changes of innate immunity and adaptive immunity in the early stage of infection. Methods five healthy Chinese rhesus monkeys were inoculated intravenously with SIVmac239 virus strain. Blood routine examination, plasma viral load and peripheral blood lymphocyte subsets were detected before infection, 2 weeks and 10 weeks after infection, respectively. Lymph node CD4 cells were observed by immunohistochemical staining and serum CD4 was detected by enzyme linked immunosorbent assay (Elisa). Results after infection, the ratio of CD4 cells to CD8 cells was decreased by 27.3% than that before infection. The ratio of P0.05 cells was increased by 44.3%, the absolute number of CD4 cells in peripheral blood decreased by 23.6T, and the CD4 cells of lymph nodes increased by 14.9B, but the difference was not statistically significant. The level of serum MBL decreased to 24.5g / 10 weeks after infection (P 0.01). Conclusion the inherent immune damage in acute infection of SIV is significant, and the imbalance between humoral immunity and cellular immunity is the main manifestation of adaptive immunity.
【作者单位】：广州中医药大学热带医学研究所;
【基金】：国家科技重大专项资助项目(2014ZX10005001) 国家自然科学基金项目(81173436);国家自然科学基金项目(81302897)~~
【分类号】：R512.91;R-332

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