小鼠骨肉瘤模型构建的关键

发布时间：2018-06-03 13:29

本文选题：Ch + 骨肉瘤　；参考：《中国比较医学杂志》2016年01期

【摘要】：目的探讨骨肉瘤动物模型荷瘤成功率的关键因素。方法 24只4~8周龄C3h小鼠,其中C3h/He J型6只、C3h/He N型18只,随机分为2组。A组采用一般脱毛膏脱毛后外侧腋下注射浓度为5×10~7个数量级/m L的LM8细胞悬液0.2 m L;B组采用动物剃毛器剃毛后外侧腋下皮下注射浓度为5×10~7个数量级/m L的LM8细胞悬液0.2 m L后每日给于注射处局部按摩,并分别记录肿瘤生长情况。结果 A组成瘤率33%(4/12);B组成瘤率100%。结论不同的注射技巧对LM8的荷瘤过程及后期护理对成瘤率具有重要的意义。
[Abstract]:Objective to investigate the key factors of success rate of osteosarcoma animal model. Methods 24 C3h mice aged 4 weeks and 8 weeks old were included in this study. Among them, 6 of C3h/He J type were C3h / He-N type, 18 of them were C3h / He-N type. Group A was randomly divided into 2 groups. Group A was injected with 5 脳 10 ~ 7 orders of magnitude / mL of LM8 cell suspension at the lateral axillary level of 5 脳 10 ~ 7 orders of magnitude / mL in group A. Group B was given 5 脳 10 ~ 7 orders of magnitude subaxillary injection with animal shaving device. 0.2ml of LM8 cell suspensions were given to local massage at the injection site daily. Tumor growth was recorded. Results the tumor rate of A was 33% and the tumor rate of B was 100%. Conclusion different injection techniques are of great significance to tumor bearing process and later stage nursing of LM8.
【作者单位】：三峡大学第一临床医学院宜昌市中心人民医院脊柱外科/脊柱微创中心脊柱医学与创伤研究所;三峡大学医学院;
【基金】：国家自然科学基金(81302346) 宜昌市科技研究基金(A12301-06) 宜昌市中心人民医院基金(KFJ2011009)
【分类号】：R-332;R738.1

【相似文献】