CV-A16和EV-A71感染对肠道上皮细胞间连接分子排布和表达的影响

发布时间：2018-06-04 09:40

本文选题：柯萨奇病毒A组型(CV-A) + 肠道病毒型(EV-A)　；参考：《病毒学报》2017年06期

【摘要】：柯萨奇病毒A组16型(Coxsakievirus A 16,CV-A16)与肠道病毒71型(Enterovirus 71,EV-A71)是引发手足口病(Hand foot and mouth disease,HFMD)的两种主要病原体,然而两者的致病机理仍未完全被阐明。病毒感染对肠道上皮细胞间连接分子排布和表达直接与病毒感染造成的后果相关。本研究通过右旋糖苷穿透实验、免疫荧光以及蛋白免疫印迹等技术检测了CV-A16和EV-A71感染对肠道上皮细胞系(Intestinal epithelial cell)FHC后细胞通透性的变化以及细胞间连接分子排布和表达情况。研究结果表明,CV-A16和EV-A71感染FHC可导致其通透性显著增强、连接分子Nectin1、Claudin4、Claudin5、ZO-1以及E-Cadherin的排布被破坏,且表达量显著性降低。这一结果提示了CV-A16和EV-A71感染肠道FHC细胞并在细胞中复制引起肠道上皮细胞损伤,导致肠道上皮细胞间连接分子排布的改变和表达量的减少。
[Abstract]:The Coxsackie virus group A 16 (Coxsakievirus A 16, CV-A16) and enterovirus 71 (Enterovirus 71, EV-A71) are the two main pathogens causing hand foot and mouth disease (Hand foot and mouth disease, HFMD). However, the pathogenesis of the two is still not fully elucidated. Virus infection is directly related to the arrangement and expression of intercellular molecules in intestinal epithelial cells. In this study, the changes in the permeability of CV-A16 and EV-A71 infection to the intestinal epithelial cell line (Intestinal epithelial cell) FHC and the arrangement and expression of intercellular connections were detected by dextran penetration test, immunofluorescence and protein immunoblotting. The results showed that CV- The infection of A16 and EV-A71 to FHC can lead to a significant enhancement of its permeability. The arrangement of Nectin1, Claudin4, Claudin5, ZO-1 and E-Cadherin is destroyed and the expression is significantly reduced. This results suggest that CV-A16 and EV-A71 infect intestinal FHC cells and cause intestinal epithelial cell damage in the cells, leading to intestinal epithelial cells. The changes in the arrangement of the molecules and the decrease of the amount of expression.
【作者单位】：中国医学科学院&北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室;
【基金】：中国医学科学院医学与健康科技创新工程重大协同创新项目(项目号:2016-I2M-1-014);题目:重要新发突发传染病生物安全基础与防控技术研究国家自然科学基金面上项目(项目号:81373142);题目:肠道病毒71型感染相关的免疫分子转录组学研究北京协和医学院协和青年科研基金项目(项目号:2017310039);题目:miR-1303靶向调控MMP9参与EV71和CA16感染中血脑屏障通透性的改变~~
【分类号】：R373

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