平冲降逆方对裸鼠人子宫膜异位症模型凋亡及TNF-α、IL-8表达的影响

发布时间：2018-06-27 00:23

本文选题：子宫内膜异位症 + 平冲降逆方　；参考：《中国中西医结合杂志》2017年08期

【摘要】：目的观察平冲降逆方对裸鼠人子宫内膜异位症(endometriosis,EMs)模型种植灶凋亡、TNF-α、IL-8表达的影响。方法将48只BALB/c-nu雌性裸鼠,随机分为两组:EMs源性模型组和非EMs源性模型组,每组24只。EMs源性模型组采用EMs患者的子宫内膜种植模型鼠;非EMs源性模型组采用非EMs患者的子宫内膜种植模型鼠。两组再分别随机分为中药组、阳性药物组和阴性对照组,每组8只。分别给予平冲降逆方、米非司酮及等量生理盐水灌胃给药4周。观察模型鼠种植灶组织学形态及超微结构、细胞凋亡、TNF-α、IL-8表达。结果 EMs及非EMs模型鼠的中药组均见病灶缩小、周围血管生成少于同源阴性对照组,光镜、电镜下可见内膜组织退变,有凋亡现象发生,免疫组化结果示凋亡率明显高于同源阴性对照组,而TNF-α、IL-8表达量明显低于同源阴性对照组(P0.05)。结论平冲降逆方可明显抑制裸鼠异位内膜的生长,这种作用可能是通过诱导细胞凋亡、降低TNF-α、IL-8表达而实现的。
[Abstract]:Objective To observe the effect of Ping Chong and descending Recipe on the apoptosis, TNF- alpha and IL-8 expression of endometriosis (EMs) model in nude mice. Methods 48 BALB/c-nu female nude mice were randomly divided into two groups: EMs source model group and non EMs model group, 24.EMs derived model groups in each group adopted the endometrial implant model of EMs patients. The non EMs source model group used the non EMs endometrium model rats. The two groups were randomly divided into the Chinese medicine group, the positive drug group and the negative control group, with 8 rats in each group. The group was given the square flushing and the descending side, mifepristone and the same amount of normal saline for 4 weeks. Death, TNF- alpha, IL-8 expression. Results both EMs and non EMs model rats were found to reduce the lesion and the peripheral blood vessels were less than the homologous negative control group. Light microscopy and electron microscopy showed that the intima tissue degeneration and apoptosis occurred. The immunohistochemical results showed that the apoptosis rate was significantly higher than that of the homologous negative control group, and the expression of TNF- alpha and IL-8 was significantly lower than that of the homologous negative group. Sex control group (P0.05). Conclusion Ping Chong and descending recipe can obviously inhibit the growth of ectopic endometrium in nude mice. This effect may be achieved by inducing apoptosis and reducing the expression of TNF- alpha and IL-8.
【作者单位】：江西中医药大学第二附属医院;江西中医药大学中医妇产科研究所;江西中医药大学研究生院;江西中医药大学附属医院妇产科;
【基金】：国家自然科学基金资助项目(No.81160447) 江西省卫生厅资助项目(No.2013A172)
【分类号】：R285.5;R-332

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