2013-2014年福州市环境污水应用于肠道病毒监测结果分析

发布时间：2018-10-23 21:10

【摘要】：目的了解环境污水监测对肠道病毒(Enterovirus,EV)相关疾病防控的意义和作用。方法选择福州祥板和洋里两个污水处理厂作为监测点,每月采集进口污水各1份,应用阴离子膜进行污水浓缩,接种RD、L20B和HEp-2进行病毒分离,微量血清中和试验及RT-PCR确定其血清型,VP1测序遗传特征分析。结果 2013-2014年采集的48份污水标本中44份(91.67%)病毒分离阳性,分离到268株病毒,已鉴定涵盖22个血清型;病毒分离高峰期为2-7月,较监测病例高峰早约2个月。2013年埃坷病毒(Echovirus,Echo)7型为优势血清型(43.61%),2014年转变为Echo6占绝对优势(62.96%);遗传特征分析显示:Echo6和Echo7污水分离株较同时期病例分离株更为多样;病例株与污水株具高度同源性,在遗传进化树图位于同一个分支。结论环境污水应用于肠道病毒监测,较现有的病例监测更为敏感。
[Abstract]:Objective to investigate the significance and role of environmental sewage monitoring in the prevention and control of enterovirus (Enterovirus,EV) related diseases. Methods two sewage treatment plants, Xiangban and Yangli in Fuzhou, were selected as monitoring sites. One sample of imported sewage was collected each month, and the anion membrane was used to concentrate the wastewater. RD,L20B and HEp-2 were inoculated to isolate the virus. Microserum neutralization test and RT-PCR were used to determine the serotype and VP1 sequencing. Results out of 48 sewage samples collected in 2013-2014, 44 (91.67%) were positive for virus isolation, 268 strains were isolated, and 22 serotypes were identified. The peak period of virus isolation was 2 to 7 months. In 2013, Echovirus,Echo virus (43.61%) was the dominant serotype (43.61%) and transformed into Echo6 in 2014 (62.96%), and the genetic characteristics analysis showed that Echo6 and Echo7 sewage isolates were more diverse than those in the same period. The case strain is highly homologous with the sewage strain and is located in the same branch of the genetic evolution tree. Conclusion Environmental sewage is more sensitive to enterovirus surveillance than existing cases.
【作者单位】：福建省疾病预防控制中心福建省人兽共患病重点实验室;福建医科大学公共卫生学院教学基地;福建医科大学公共卫生学院;
【基金】：福建省卫生系统中青年骨干人才培养项目(2013-ZQN-ZD-10)~~
【分类号】：R123.1;R373.2

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