电针对特应性皮炎瘙痒模型小鼠的止痒作用及对强啡肽、P物质的影响

发布时间：2018-11-11 11:15

【摘要】：目的观察电针不同穴位对特应性皮炎(AD)瘙痒模型小鼠搔抓行为、脊髓强啡肽(DYN)及血清P物质(SP)的影响。方法将32只雌性BALB/c小鼠分为空白组、模型组、内关组和曲池组,每组8只。除空白组外,其余各组均以2,4-二硝基氯苯(DNCB)诱导建立AD动物模型,共刺激7次。内关组和曲池组在实验第5天开始分别给予电针内关、曲池穴治疗。空白组和模型组不给予任何治疗。观察小鼠搔抓次数、腹部皮损改变,运用Western印迹法检测脊髓DYN含量,ELISA法检测血清中SP水平变化。结果与空白组比较,模型组小鼠搔抓次数显著升高,腹部皮肤产生AD样湿疹皮损改变,脊髓内DYN及血清SP水平明显升高(P0.05,P0.01)。与模型组比较,内关组和曲池组小鼠的搔抓次数显著降低,腹部皮损炎症略有减轻,脊髓DYN含量明显升高(均P0.05)、血清SP水平显著降低(P0.05);内关组和曲池组间比较,各指标均未见统计学差异(P0.05)。结论电针对DNCB诱发的AD样瘙痒模型小鼠的止痒作用可能是通过提高脊髓中DYN的含量、抑制SP水平而介导的。
[Abstract]:Objective to observe the effects of electroacupuncture at different points on scratching behavior, dynorphin (DYN) and substance P (SP) in mice with atopic dermatitis (AD). Methods 32 female BALB/c mice were divided into three groups: blank group, model group, Neiguan group and Quchi group. With the exception of the blank group, the other groups were induced to establish the AD animal model by (DNCB) for 7 times. Neiguan group and Quchi group were treated with electroacupuncture at Neiguan and Quchi points respectively on the fifth day of the experiment. Blank and model groups were not given any treatment. The number of scratches and the changes of abdominal lesions in mice were observed. The content of DYN in spinal cord was detected by Western blot and the level of SP in serum was detected by ELISA method. Results compared with the blank group, the number of scratches in the model group was significantly increased, the lesions of AD like eczema in abdominal skin were changed, and the levels of DYN and serum SP in the spinal cord were significantly increased (P0.05, P0.01). Compared with the model group, the number of scratches in Neiguan group and Kuchi group was significantly decreased, the inflammation of abdominal lesions was slightly reduced, the content of DYN in spinal cord was significantly increased (P0.05), and the level of serum SP was significantly decreased (P0.05). There was no statistical difference between Neiguan group and Quchi group (P0.05). Conclusion the antipruritus effect of electroacupuncture on AD-like pruritus model mice induced by DNCB may be mediated by increasing the content of DYN in spinal cord and inhibiting the level of SP.
【作者单位】：广州中医药大学第二临床医学院;
【基金】：国家自然科学基金青年基金项目(81403461)
【分类号】：R245;R-332

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