鸡回肠淋巴细胞发育的研究
发布时间：2019-02-19 08:46
【摘要】：本实验分别选择18、20胚龄鸡胚,1、4、7、10、14、21、35及56日龄的雏鸡,每个时期取10只鸡的新鲜回肠,制成冰冻切片,分别进行HE染色和免疫组织化学染色。结合图像分析和数据统计,对鸡回肠的组织结构发育变化、淋巴细胞的出现、分布及定位规律做出研究。旨在了解不同时期鸡肠道免疫细胞的发育情况,揭示鸡肠道的免疫状态和机制,为适时免疫,更好的防控禽病提供一定的参考资料。 通过HE染色方法研究鸡回肠组织结构变化情况,结果表明：1日龄时,可见回肠绒毛呈指状,肠腺较少；4日龄时,绒毛高度同1日龄相比有所降低,肠绒毛固有层增宽,绒毛相互融合形成较大的皱襞,隐窝深度增加,在回肠黏膜固有层中可见有少量的淋巴滤泡；7日龄时,绒毛高度增加,肠腺明显,隐窝深度显著增加(P0.05),超过其它日龄,达到峰值；10日龄时,绒毛形状多呈叶状,绒毛高度、淋巴滤泡及杯状细胞的增多；14日龄时,隐窝深度到达稳定水平；35日龄时,绒毛高度到达稳定水平。 通过免疫组织化学方法研究鸡回肠中T淋巴细胞及其亚群的分布定位规律,结果表明：CD3+和CD8+细胞于18胚龄时出现,CD4+于1日龄时出现。在分布定位上,CD3+细胞在回肠黏膜上皮及固有层中都有分布,CD4+以黏膜固有层中的分布为主,而CD8+T淋巴以黏膜上皮内的分布为主。在数量变化上,4日龄时,CD3+、CD4+及CD8+T淋巴细胞的数量均显著增加；10日龄时,CD3+和CD8+细胞的数量上升,CD4+细胞数量减少；14日龄时,CD3+和CD8+细胞的数量减少,CD4+细胞数量增加；21日龄时,CD3+、CD4+和CD8+T细胞数量显著增加(P0.05),到达稳定水平。 Bu-1+和IgA+细胞于18胚龄时出现,IgG+细胞于1日龄时出现。在数量变化上,从1日龄到7日龄,Bu-1+细胞的数量一直保持增加的趋势；10、14日龄时,Bu-1+细胞的数量同7日龄相比略有下降；随后,Bu-1+细胞的数量开始增加,21日龄时,Bu-1+细胞的数量同14日龄相比显著增加(P0.05)；35日龄,Bu-1+细胞的数量到达峰值。IgA+细胞的数量变化整体呈逐渐增加的趋势。18胚龄到10日龄,IgA+细胞的数量持续增加,21日龄时,IgA+细胞的数量显著增加,随后,IgA+细胞的数量略有下降,但并不显著。IgG+细胞在18胚龄到14日龄大致呈持续增加的趋势,仅在7日龄时,IgG+细胞的数量稍有下降,但并不显著。21日龄时,IgG+细胞的数量显著增加；35日龄时,IgG+细胞的数量到达峰值；56日龄时,IgG+细胞的数量同35日龄相比显著下降(P0.05)。 肠道中淋巴细胞的发育,能够促进回肠组织结构发育的完善,而肠道组织发育的一系列变化,为回肠相关免疫细胞的发育提供必须的微环境。这两者互相协调作用,对机体的黏膜免疫系统的成熟起至关重要的作用。
[Abstract]:In this experiment, the fresh ileum of 10 chickens were collected from 18 ~ 20 embryo age chicken embryos and 1 ~ 4 ~ 4 ~ 7 ~ (10) ~ 10 ~ (14) ~ 21 ~ (-35) and 56 ~ (th) days old chicks respectively. The fresh ileum of 10 chickens were made into frozen sections and stained by HE and immunohistochemistry respectively. Based on image analysis and data statistics, the changes of tissue structure, the appearance, distribution and localization of lymphocytes in chicken ileum were studied. The aim of this study was to understand the development of intestinal immune cells in different periods and to reveal the immune status and mechanism of chicken intestinal tract so as to provide some reference materials for timely immunization and better prevention and control of poultry diseases. The changes of ileum tissue structure in chicken were studied by HE staining. The results showed that the ileal villi were finger shaped and the intestinal glands were less at 1 day of age. At 4 days of age, the height of villi decreased compared with that of 1 day, the lamina propria of intestinal villi widened, the villi fused to form larger folds, and the depth of crypt increased. A small number of lymphatic follicles could be seen in the lamina propria of ileum mucosa. At the age of 7 days, the villus height, intestinal gland and crypt depth increased significantly (P0.05), which reached the peak value over the other days, and at 10 days, the villi were mostly leaf shaped, villous height, lymphatic follicles and goblet cells increased. At the age of 14 days, the depth of crypt reached stable level, and the height of villi reached stable level at 35 days of age. The distribution and localization of T lymphocytes and their subsets in chicken ileum were studied by immunohistochemical method. The results showed that CD3 and CD8 cells appeared at 18 embryo age and CD4 appeared at 1 day old. In distribution localization, CD3 cells were distributed in ileal mucosa epithelium and lamina propria, CD4 was mainly distributed in lamina propria mucosa, and CD8 T lymphoid cells were mainly distributed in mucosal epithelium. The number of CD3, CD4 and CD8 T lymphocytes increased significantly at 4 days of age, and the number of CD3 and CD8 cells increased and the number of CD4 cells decreased at 10 days of age. At the age of 14 days, the number of CD3 and CD8 cells decreased and the number of CD4 cells increased, while at 21 days of age, the number of CD3, CD4 and CD8 T cells increased significantly (P0.05), reaching a stable level. Bu-1 and IgA cells appeared at 18 embryo age and IgG cells appeared at 1 day old. The number of Bu-1 cells increased from day 1 to day 7, and the number of Bu-1 cells decreased slightly at the age of 10 ~ 14 days. Then, the number of Bu-1 cells began to increase, and the number of Bu-1 cells increased significantly at 21 days old compared with 14 days old (P0.05). At the age of 35 days, the number of Bu-1 cells reached its peak. The number of IgA cells increased gradually. The number of IgA cells continued to increase from 18 to 10 days of age, and the number of IgA cells increased significantly at 21 days of age. The number of IgA cells decreased slightly, but not significantly. The number of IgG cells increased from 18 embryo to 14 days old, only at 7 days old, the number of IgG cells decreased slightly, but not significantly at 21 days old. The number of IgG cells increased significantly. At the age of 35 days, the number of IgG cells reached the peak; at the age of 56 days, the number of IgG cells decreased significantly compared with the age of 35 days (P0.05). The development of lymphocytes in the intestine can promote the development of the ileal tissue structure, and a series of changes of the intestinal tissue development provide the necessary microenvironment for the development of the ileal-associated immune cells. The coordination of the two plays an important role in the maturation of the mucosal immune system.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R392
本文编号：2426326
[Abstract]:In this experiment, the fresh ileum of 10 chickens were collected from 18 ~ 20 embryo age chicken embryos and 1 ~ 4 ~ 4 ~ 7 ~ (10) ~ 10 ~ (14) ~ 21 ~ (-35) and 56 ~ (th) days old chicks respectively. The fresh ileum of 10 chickens were made into frozen sections and stained by HE and immunohistochemistry respectively. Based on image analysis and data statistics, the changes of tissue structure, the appearance, distribution and localization of lymphocytes in chicken ileum were studied. The aim of this study was to understand the development of intestinal immune cells in different periods and to reveal the immune status and mechanism of chicken intestinal tract so as to provide some reference materials for timely immunization and better prevention and control of poultry diseases. The changes of ileum tissue structure in chicken were studied by HE staining. The results showed that the ileal villi were finger shaped and the intestinal glands were less at 1 day of age. At 4 days of age, the height of villi decreased compared with that of 1 day, the lamina propria of intestinal villi widened, the villi fused to form larger folds, and the depth of crypt increased. A small number of lymphatic follicles could be seen in the lamina propria of ileum mucosa. At the age of 7 days, the villus height, intestinal gland and crypt depth increased significantly (P0.05), which reached the peak value over the other days, and at 10 days, the villi were mostly leaf shaped, villous height, lymphatic follicles and goblet cells increased. At the age of 14 days, the depth of crypt reached stable level, and the height of villi reached stable level at 35 days of age. The distribution and localization of T lymphocytes and their subsets in chicken ileum were studied by immunohistochemical method. The results showed that CD3 and CD8 cells appeared at 18 embryo age and CD4 appeared at 1 day old. In distribution localization, CD3 cells were distributed in ileal mucosa epithelium and lamina propria, CD4 was mainly distributed in lamina propria mucosa, and CD8 T lymphoid cells were mainly distributed in mucosal epithelium. The number of CD3, CD4 and CD8 T lymphocytes increased significantly at 4 days of age, and the number of CD3 and CD8 cells increased and the number of CD4 cells decreased at 10 days of age. At the age of 14 days, the number of CD3 and CD8 cells decreased and the number of CD4 cells increased, while at 21 days of age, the number of CD3, CD4 and CD8 T cells increased significantly (P0.05), reaching a stable level. Bu-1 and IgA cells appeared at 18 embryo age and IgG cells appeared at 1 day old. The number of Bu-1 cells increased from day 1 to day 7, and the number of Bu-1 cells decreased slightly at the age of 10 ~ 14 days. Then, the number of Bu-1 cells began to increase, and the number of Bu-1 cells increased significantly at 21 days old compared with 14 days old (P0.05). At the age of 35 days, the number of Bu-1 cells reached its peak. The number of IgA cells increased gradually. The number of IgA cells continued to increase from 18 to 10 days of age, and the number of IgA cells increased significantly at 21 days of age. The number of IgA cells decreased slightly, but not significantly. The number of IgG cells increased from 18 embryo to 14 days old, only at 7 days old, the number of IgG cells decreased slightly, but not significantly at 21 days old. The number of IgG cells increased significantly. At the age of 35 days, the number of IgG cells reached the peak; at the age of 56 days, the number of IgG cells decreased significantly compared with the age of 35 days (P0.05). The development of lymphocytes in the intestine can promote the development of the ileal tissue structure, and a series of changes of the intestinal tissue development provide the necessary microenvironment for the development of the ileal-associated immune cells. The coordination of the two plays an important role in the maturation of the mucosal immune system.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R392
【参考文献】
相关期刊论文 前10条
1 韩纯;T细胞亚群的分类及其功能进展(综述)[J];安徽卫生职业技术学院学报;2003年03期
2 王丽萍，，刘玉堂，肖向红，邹红非，王永林;环颈雉消化系统组织形态学观察[J];动物学杂志;1994年03期
3 张立世;杨树宝;姜云垒;栾维民;;鸡盲肠扁桃体组织结构早期发育的动态观察[J];家禽科学;2011年04期
4 汪道鑫;虞春华;叶颖俊;汪炜;;T淋巴细胞的研究进展[J];第四军医大学学报;2009年24期
5 董玉兰,王树迎;动物黏膜免疫细胞研究进展[J];动物医学进展;2003年01期
6 计慧琴,王丙云,陈志胜,江青艳;T细胞的发育分化及胸腺激素的作用[J];动物医学进展;2005年04期
7 丁轲;余祖华;王天奇;;肠道黏膜免疫研究进展[J];动物医学进展;2007年10期
8 邱梅平;王恬;;低磷日粮中添加植酸酶对蛋鸡肠道形态结构的影响[J];饲料工业;2009年24期
9 祁保民;刘洪娜;;番鸭肠道相关淋巴组织的分布和组织学结构[J];福建农业大学学报;2006年04期
10 王彩虹,李安兴,佘锐萍;鸡肠道免疫的研究进展[J];国外畜牧科技;2001年05期
相关会议论文 前1条
1 高春生;徐亚平;白晓辉;袁文菊;肖传斌;;父母代固始鸡回肠发育形态学研究[A];中国畜牧兽医学会动物解剖学及组织胚胎学分会第十四次学术研讨会论文集[C];2006年
相关博士学位论文 前2条
1 徐鹏辉;肠黏膜上皮细胞与免疫细胞相互作用的研究[D];中国人民解放军军事医学科学院;2005年
2 栾维民;鸡外周免疫器官中淋巴细胞发育的研究[D];吉林农业大学;2008年
相关硕士学位论文 前1条
1 赵永晓;慢性HBV感染不同阶段细胞免疫及其与病毒清除的关系[D];河北医科大学;2009年
本文编号：2426326
本文链接：https://www.wllwen.com/xiyixuelunwen/2426326.html
最近更新
教材专著
