蝙蝠圆环病毒BtCV-DS13株的鉴定与全基因组分析

发布时间：2019-03-12 21:02

【摘要】：自首次从棕果蝠(Rousettus leschenaultii)中检测到圆环病毒后,不断有研究小组从不同地域的多种蝙蝠体内发现类似病毒。为掌握中国东部沿海蝙蝠圆环病毒的流行病学资料,以浙江省舟山地区采集的大卫鼠耳蝠的肠道组织为模板,采用巢式PCR和染色体步移法相结合的方法获得一株蝙蝠圆环病毒的全基因组序列,并命名为BtCV-DS13。序列分析表明,BtCV-DS13全基因组长度为1 873nt,具有圆环病毒的典型基因组结构特征。同源性比较显示,与已公布的4种蝙蝠来源的圆环病毒(Bat1、Bat2、Bat3)或环病毒代表株(Cyclovirus bat)的同源性均较低,分别为22.9%、53.5%、24.7%和4.5%。系统进化分析显示,BtCV-DS13处于圆环病毒属的一个大分支中,与猪圆环病毒和蝙蝠圆环病毒处于其中同一个小分支。基于以上分析,初步判断BtCV-DS13在分类上是一种新的蝙蝠圆环病毒。
[Abstract]:Since the first detection of circovirus from the (Rousettus leschenaultii) of the brown fruit bat, teams have been discovering similar viruses from a variety of bats in different regions. In order to master the epidemiological data of bat circovirus in the eastern coast of China, the intestinal tissue of the bats collected in Zhoushan area, Zhejiang Province, was used as a template. The whole genome sequence of bat circovirus was obtained by means of nested PCR and chromosome stepping method. It was named BtCV-DS13.. Sequence analysis showed that the whole genome length of BtCV-DS13 was 1873 NT and had typical genome structure characteristics of circovirus. The homology comparison showed that the homology between Bat1,Bat2,Bat3 and (Cyclovirus bat) was 22.9%, 53.5%, 24.7% and 4.5%, respectively. Phylogenetic analysis showed that BtCV-DS13 was in a large branch of the genus circovirus and was in the same small branch as porcine circovirus and bat circovirus. Based on the above analysis, it is concluded that BtCV-DS13 is a new bat circovirus in classification.
【作者单位】：中国人民解放军第三军医大学;南京军区军事医学研究所;
【基金】：军队后勤科研计划项目(项目号:AWS16Z023,AWS11C009,BWS14J025,CNJ16C008) 国家重大传染病专项(项目号:2013ZX10004103-004);题目:重要病毒性出血热及虫媒传染病病原体检测方案的建立与验证全国博士后特别资助课题(项目号:2016T91001);题目:东南沿海屯兵坑道蝙蝠携带重要人畜共患病病毒研究江苏省博士后课题(编号:1501147C);题目:舟山群岛蝙蝠携带重要人畜共患病病毒研究
【分类号】：R373

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