单纯腹壁高能爆炸伤合并内脏外露的动物模型的建立与实验观察研究

发布时间：2019-04-09 19:10

【摘要】：目的：为致伤后较长时间的观察单纯腹壁爆炸伤合并内脏器官外露的伤情，研究此类创伤的致伤和转归规律，，建立一种能在致伤后较长时间存活的单纯腹壁爆炸伤合并内脏器官外露的动物模型。方法： 8-10周龄，生长期白家兔12只，随机分为2组，每组6只。在全身麻醉条件下，预先备皮标记致伤部位。实验组采用自制腹腔防护装置置入腹腔，然后采用0.125gTNT当量炸药球贴于致伤部位致伤；对照组不防护处理直接采用同样爆源致伤。伤后观察各组实验动物大体形态，对活体动物每日取伤部组织行病理切片观察，对死亡动物记录生存时间并行尸检。结果：两组动物致伤部位腹壁均呈全层开放性爆炸伤。实验组实验动物创面面积为3.8±0.56cm2；对照组创伤面积为3.93±0.49cm2。P0.05两组间并无显著差异。实验组实验动物存活时间为142±87.78h。对照组实验动物平均存活时间为3.77±2.25h。P0.05。两组动物存活时间存在明显差异。实验组明显长于对照组。尸检和病理观察发现，对照组动物早期死亡原因为腹腔内出血和内脏损伤，实验组则发生内脏纤维粘连，但未见明显内脏创伤。病理组织切片观察发现，腹壁各层组织受爆炸创伤有差异，呈现“外轻内重”的病理变化，并进行性发展，肌肉组织的变化最为典型。结论：本实验模拟了单纯腹壁爆炸伤合并内脏器官外露的致伤机制。经有效防护，建立了致伤后存活时间较长的单纯腹壁爆炸伤动物模型。
[Abstract]:Objective: in order to observe the injury condition of abdominal wall explosion injury complicated with visceral organ exposure for a long time after injury, to study the regularity of injury and prognosis of this kind of trauma. An animal model of abdominal blast injury complicated with visceral organ exposure was established, which could survive for a long time after injury. Methods: twelve rabbits were randomly divided into two groups, 6 rabbits in each group. Under the condition of general anesthesia, prepare skin marking wound site in advance. The experimental group was treated with self-made celiac protective device, then the 0.125gTNT equivalent explosive ball was applied to the injured site, and the control group was directly injured by the same explosive source without protective treatment. The gross morphology of experimental animals in each group was observed after injury. Pathological sections were observed on the injured tissues of living animals every day. The survival time of dead animals was recorded and autopsies were performed. Results: the abdominal wall of both groups showed open blast injury. The wound area of the experimental group was 3.8 卤0.56 cm 2, while that of the control group was 3.93 卤0.49cm2.P0.05. There was no significant difference between the two groups. The survival time of experimental group was 142 卤87.78 hours. The average survival time of the experimental animals in the control group was 3.77 卤2.25h. P 0.05. There was significant difference in survival time between the two groups. The experimental group was significantly longer than the control group. Postmortem and pathological observation showed that the causes of early death in the control group were abdominal hemorrhage and visceral injury, while visceral fiber adhesion occurred in the experimental group, but no obvious visceral trauma was found in the experimental group. Pathological sections showed that there were some differences in the explosive trauma of the abdominal wall, showing the pathological changes of "external light and internal weight", and progressive development. The change of muscle tissue was the most typical. Conclusion: the mechanism of abdominal wall explosion injury complicated with visceral organ exposure was simulated in this study. The animal model of simple abdominal wall explosion injury with long survival time after injury was established by effective protection.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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