当归补血汤对慢性轻度不可预见性刺激抑郁模型大鼠行为学及HPA轴的影响

发布时间：2019-04-29 07:44

【摘要】：目的:观察当归补血汤对慢性轻度不可预见性刺激郁模型大鼠行为学和下丘脑-垂体-肾上腺皮质轴(HPA轴)的影响。方法:将120只SD雄性大鼠随机分为正常组、模型组、当归补血汤高、中、低剂量组(8,4,2 g·kg-1)和阳性药组(盐酸氟西汀,3.33 mg·kg-1)。除正常组外,其余各组采用慢性轻度不可预见性刺激法(CUMS)制备抑郁大鼠模型。各给药组动物灌胃给相应药物,正常组和模型组大鼠灌胃给予等体积生理盐水;每日1次,连续21 d。分别于第0,7,14,21天采用强迫游泳实验、悬尾实验和旷场实验等方法对抑郁模型大鼠的行为学改变进行检测,并于21 d采用酶联免疫吸附测定法(ELISA)检测大鼠血清促肾上腺皮质激素(ACTH)和皮质酮(CORT)水平,采用实时荧光定量聚合酶链式反应法(Real-time PCR)检测大鼠下丘脑促肾上腺皮质激素释放激素(CRH),下丘脑、垂体和海马糖皮质激素受体(GR)mRNA表达水平。结果:当归补血汤高、中剂量组可显著减少抑郁模型大鼠强迫游泳实验和悬尾实验的不动时间(P0.05);显著增多旷场实验穿格数次数(P0.05);显著降低血清CORT和ACTH水平(P0.05);当归补血汤高剂量组可下调CRH mRNA表达水平;上调下丘脑、垂体和海马的GR mRNA的表达水平(P0.05)。结论:当归补血汤具有改善CUMS大鼠抑郁行为的作用,表现出抗抑郁作用,其调节HPA轴功能是抗抑郁作用的重要机制之一。
[Abstract]:Aim: to observe the effect of Danggui Buxue decoction on behavior and hypothalamus-pituitary-adrenal cortex axis (HPA axis) in rats with chronic mild unpredictable irritating depression. Methods: 120 male SD rats were randomly divided into normal group, model group, high, middle, low dose group (8,4,2 g kg-1) and positive group (fluoxetine hydrochloride, 3.33 mg kg-1). In addition to the normal group, chronic mild unpredictable stimulation (CUMS) was used to establish the depression rat model. Rats in the normal group and the model group were given the same volume of saline, once a day, for 21 days, and the rats in the normal group and the model group were given the same volume of normal saline, once a day for 21 days. The behavioral changes of depression model rats were detected by forced swimming test, tail suspension test and open field test on the 0,7,14,21 days respectively. The levels of serum corticotropin (ACTH) and corticosterone (CORT) were measured by enzyme-linked immunosorbent assay (ELISA) on day 21. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of glucocorticoid receptor (GR) mRNA (GR) mRNA) in hypothalamus, pituitary and hippocampus of rat hypothalamic corticotropin-releasing hormone (CRH),). Results: Danggui Buxue decoction was high and middle dose group could significantly reduce the immobility time of forced swimming test and tail suspension test in depression model rats (P0.05), and significantly increase the number of traversing times in open field experiment (P0.05). The levels of serum CORT and ACTH were significantly decreased (P0.05), the expression of CRH mRNA was down-regulated and the expression of GR mRNA in hypothalamus, pituitary and hippocampus was up-regulated (P0.05) in the high dose group of Danggui Buxue decoction. Conclusion: Danggui Buxue decoction can improve the depressive behavior of CUMS rats and has antidepressant effect. The regulation of HPA axis function is one of the important mechanisms of antidepressant action.
【作者单位】：山西中医学院;山西省中西医结合医院;
【基金】：国家“十二五”科技支撑计划项目(2011BAI07B01)
【分类号】：R285.5;R-332

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