淫羊藿醇提物对高脂血症模型小鼠的降血脂作用及其抗氧化活性

发布时间：2019-06-12 11:12

【摘要】：目的:探讨淫羊藿醇提物(EEBM)对高脂血症模型小鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平的影响,阐明其抗氧化作用。方法:采用铁离子还原力法、2,2-二苯基苦基肼(DPPH)自由基清除法测定EEBM的抗氧化活性。建立高血脂小鼠模型,将小鼠随机分为正常对照组、模型组、阳性对照组和低、中、高剂量EEBM组,分别采用预防性给药和治疗性给药28和42d。实验结束后,分别测定小鼠血清TC、TG、LDL-C和HDL-C水平。结果:体外抗氧化实验,EEBM对DPPH自由基清除活力的半数抑制率(IC50)为(35.12±0.48)mg·L~(-1),对铁离子还原的半最大效应浓度(EC50)为(148.35±1.38)mg·L~(-1)。降血脂活性,无论是预防性给药还是治疗性给药,与模型组比较,低、中和高剂量EEBM组小鼠血清TC、TG和LDL-C水平均明显降低(P0.05或P0.01),HDL-C水平均明显升高(P0.01)。结论:EEBM可改变高脂血症小鼠血脂质水平使之接近正常水平,EEBM具有明显的降血脂活性。
[Abstract]:Aim: to investigate the effect of alcohol extract (EEBM) of icariae on serum total cholesterol (TC), (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in hyperlipidemia model mice. Methods: the antioxidant activity of EEBM was determined by iron ion reduction force method and 2, 2-diphenylpicarbazide (DPPH) radical scavenging method. The hyperlipidemia mice were randomly divided into normal control group, model group, positive control group and low, middle and high dose EEBM groups. The mice were given prophylactic administration and therapeutic administration for 28 and 42 days, respectively. At the end of the experiment, the levels of serum TC,TG,LDL-C and HDL-C in mice were measured. Results: in vitro antioxidant test, the half inhibition rate (IC50) of EEBM on DPPH free radical scavenging activity was (35.12 卤0.48) mg 路L ~ (- 1), and the semi-maximum effect concentration (EC50) on iron ion reduction was (148.35 卤1.38) mg 路L ~ (- 1). Compared with the model group, the levels of serum TC,TG and LDL-C in the low, middle and high dose EEBM groups were significantly lower than those in the model group (P 0.05 or P 0.01), and the levels of HDL-C were significantly higher than those in the model group (P 0.01). Conclusion: EEBM can change the blood lipid level of hyperlipidemia mice and make it close to the normal level. EEBM has obvious lipid-lowering activity.
【作者单位】：吉林农业大学中药材学院中药化学教研室;
【基金】：国家科技部科技支撑计划项目资助课题(2011BAI03B01)
【分类号】：R285.5;R-332

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