广西红水河流域长寿人群CETP TaqIB和I405V基因多态性与血脂和长寿相关性的研究
发布时间：2019-06-22 20:05
【摘要】：背景： 胆固醇酯转移蛋白(cholesteryl ester transfer protein, CETP)在胆固醇逆向转运中发挥关键作用,是脂类代谢中的重要调节蛋白。CETP是血浆HDL-C水平的最重要的决定因素之一,CETP基因发生改变可能引起血浆HDL-C水平的改变从而影响HDL和LDL的颗粒大小、血浆浓度,影响脂质代谢,使心血管疾病的危险性升高,继而影响寿命。CETP基因的多态性与血脂水平及长寿关系的研究已有一些报道,但目前尚未取得一致性的结论。 目的： 本研究主要探讨广西红水河流域长寿人群CETP基因TaqIB和1405V位点的多态性,并探讨其基因型与该地区血脂水平和长寿的关系。 方法： 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对广西红水河流域90岁以上壮族长寿老人523例(长寿组),普通健康壮族中老年人498例(对照组)进行CETP TaqIB和1405V位点的基因分型,并分析各基因型对血压、体重指数(BMI)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)等指标的影响,了解CETPTaqIB和I405V多态性与该群体血脂水平的关系及其在该地区长寿中的可能作用。 结果： 1.一般情况及血脂水平：长寿组血压水平、高血压率、TC、HDL-C、 LDL-C水平显著高于对照组；而BMI、TG水平及血脂异常率低于对照组。 2.基因分型：CETP TaqIB等位基因在两组均以B1为主,频率60%；基因型则以野生型B1B1及杂合型B1B2为主。CETPI405V等位基因在两组均以Ⅰ为主,约占58%,基因型以野生型Ⅱ和杂合型Ⅳ为主。CETP TaqIB和I405V两位点的基因型频率分布及等位基因频率分布在长寿组和对照组间均未发现有显著差异。但区分性别后组间比较分析显示,对照组女性TaqIB B2等位基因频率明显低于长寿组女性(33.3vs39.1,P0.05)；在I405V位点,对照组男性和女性在基因型频率分布方面存在显著差异(p=0.008),大体表现为女性ⅤⅤ和Ⅱ基因型频率高于男性(分别为20.4vs12.6,37.6vs32.4),而女性Ⅳ则低于男性(42.0vs54.9)。 3.基因型与BMI的关系：各组进一步区分为BMI24kg/m2(正常体型)和≥24kg/m2(超重)亚组,发现对照组超重率显著高于长寿组(24.1%vs12.4%,P0.05)。在Taq IB位点,各组没发现不同BMI在基因型频率及等位基因频率上的差异；而在I405V位点上,对照组超重者基因型ⅤⅤ及Ⅱ的频率高于非超重者,而ⅣV则低于非超重者(P=0.011)。 4.基因型与血脂水平的关系：在Taq IB位点,组内比较显示,基因型为B2B2和B1B2的HDL-C水平均明显高于B181的HDL-C水平(P0.05)。组间比较发现,长寿组B1B1携带者的HDL-C水平及各种基因型的LDL-C水平显著高于对照组(P0.05),区分性别后,长寿组男性的这种表现仍存在。在I405V位点,组内比较显示各血脂水平在不同基因型间无显著差异,然而,组间比较发现,对照组男性Ⅱ基因型的TC和LDL-C水平有高于Ⅳ和VV的趋势,而女性无此差异。 5.线性回归分析：合并人群的TC、TG水平与舒张压、BMI呈正显著相关(P0.01),与年龄呈负相关(P0.01～0.05),HDL-C水平与年龄、Taq IB呈显著正相关(P0.01),与BMI和I405V呈负相关(P0.01～0.05),LDL-C与舒张压呈显著正相关(P0.01)。 结论： 1.广西红水河流域长寿人群与自然人群在一些血脂水平、血脂异常率、高血压率等方面存在明显差异。 2.总体上,CETP TaqIB和I405V两位点的基因型频率及等位基因频率分布在长寿人群和自然人群间无差异。 3. CETP Taq IB B2等位基因携带者(包括基因型B2B2和B1B2)在处理胆固醇上有一定优势,主要表现为HDL-C增高。 4.红水河流域的长寿与CETP基因多态有一定的关系,并存在性别差异,可能通过某些优势基因型影响血脂水平,尤其是HDL-C的水平,从而减少冠心病等老年相关性疾病的发生,延长寿命。
[Abstract]:Background: Cholesteryl ester transfer protein (CETP) plays a key role in the reverse transport of cholesterol, and is an important regulation egg in lipid metabolism. White. CETP is one of the most important determinants of plasma HDL-C levels. The changes in the CETP gene may cause a change in the plasma HDL-C level, thus affecting the size of the particles of HDL and LDL, the plasma concentration, the lipid metabolism, the risk of cardiovascular disease, and the longevity The study of the relationship between the polymorphism of CETP gene and the level of blood lipid and long-life has been reported. On the other hand. Objective: To study the polymorphism of CETP gene TaqIB and 1405 V in the Longevity population of Hongshui River in Guangxi, and to explore its genotype and the level of blood lipid in the region. long-lived Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to study the effect of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on 523 cases (long-life group) and 498 cases (control group) of the strong and long-life old people in Hongshui River, Guangxi, and 498 cases (control group) of the general healthy and healthy people were treated with CETP TaqIB and 140. Genotyping of the 5 V locus, and the analysis of each genotype on blood pressure, body mass index (BMI), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL) -C) The effect of the indicators such as the relationship between the CETPTaqIB and the I405V polymorphism and the level of blood lipid in the population and their presence long life of the district Results:1. The level of blood pressure, blood pressure, TC, HDL-C and LDL-C in the long-life group were significantly higher than that in the control group. The level and the abnormal rate of dyslipidemia were lower than that of the control group.2. Genotyping: CETP TaqIB allele was dominant in both groups with frequency of 60%; genotype was wild The type B1B1 and the heterozygous B1B2 were the main. The CETPI405V allele was mainly in the first group and about 58% in both groups. Genotype was dominated by wild type 鈪，
本文编号：2504914
[Abstract]:Background: Cholesteryl ester transfer protein (CETP) plays a key role in the reverse transport of cholesterol, and is an important regulation egg in lipid metabolism. White. CETP is one of the most important determinants of plasma HDL-C levels. The changes in the CETP gene may cause a change in the plasma HDL-C level, thus affecting the size of the particles of HDL and LDL, the plasma concentration, the lipid metabolism, the risk of cardiovascular disease, and the longevity The study of the relationship between the polymorphism of CETP gene and the level of blood lipid and long-life has been reported. On the other hand. Objective: To study the polymorphism of CETP gene TaqIB and 1405 V in the Longevity population of Hongshui River in Guangxi, and to explore its genotype and the level of blood lipid in the region. long-lived Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to study the effect of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on 523 cases (long-life group) and 498 cases (control group) of the strong and long-life old people in Hongshui River, Guangxi, and 498 cases (control group) of the general healthy and healthy people were treated with CETP TaqIB and 140. Genotyping of the 5 V locus, and the analysis of each genotype on blood pressure, body mass index (BMI), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL) -C) The effect of the indicators such as the relationship between the CETPTaqIB and the I405V polymorphism and the level of blood lipid in the population and their presence long life of the district Results:1. The level of blood pressure, blood pressure, TC, HDL-C and LDL-C in the long-life group were significantly higher than that in the control group. The level and the abnormal rate of dyslipidemia were lower than that of the control group.2. Genotyping: CETP TaqIB allele was dominant in both groups with frequency of 60%; genotype was wild The type B1B1 and the heterozygous B1B2 were the main. The CETPI405V allele was mainly in the first group and about 58% in both groups. Genotype was dominated by wild type 鈪，
本文编号：2504914
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