脉冲射频对坐骨神经慢性压迫模型大鼠的痛觉过敏及脊髓背角NR2B亚基表达的作用
发布时间：2019-07-25 16:07
【摘要】：目的观察脉冲射频(PRF)对坐骨神经慢性压迫(CCI)后神经病理性疼痛模型大鼠疼痛行为学、损伤坐骨神经结组织病理学的影响,检测脊髓背角NR2B亚基的表达。方法 48只Sprague-Dawley大鼠随机等分为Sham-Sham(SS)组、Sham-PRF(SP)组、CCI-Sham(CS)组和CCI-PRF(CP)组。CS、CP组大鼠结扎右侧坐骨神经干,制作CCI模型;SS、SP组只分离坐骨神经而不结扎。术后15 d,CP、SP组于坐骨神经干结扎处行PRF,SS、CS组仅放置电极针不通电。造模前,造模后3 d、7 d、11 d、15 d,治疗后1 d、3 d、7 d、11 d、15 d测量大鼠右后足机械缩足阈值(HWT);治疗后15 d光镜下行右侧坐骨神经干评分;Western blotting测定L4-6脊髓背角中NR2B亚基蛋白表达。结果造模后CS、CP组HWT较SS、SP组降低;治疗后,CP组HWT高于CS组。光镜下CP组较CS组神经轴索直径、髓鞘厚度明显增加(P0.01)。CP组大鼠脊髓背角NR2B表达低于CS组(F=10.769,P0.05)。结论PRF能降低CCI模型脊髓背角NR2B亚基表达,修复受损神经,降低机械痛敏。
[Abstract]:Objective to observe the effect of pulse radiofrequency (PRF) on pain behavior and histology of injured sciatic nerve node in rats with neuropathic pain after chronic compression of (CCI), and to detect the expression of NR2B subunit in spinal dorsal horn. Methods 48 Sprague-Dawley rats were randomly divided into Sham-Sham (SS) group, Sham-PRF (SP) group, CCI-Sham (CS) group and CCI-PRF (CP) group. CS, CP group rats ligated the right sciatica trunk to make CCI model, SS,SP group only isolated the sciatica without ligating. On the 15th day after operation, PRF,SS,CS group only placed electrode needle without electricity in SP group. Before modeling, 3 days, 7 days, 15 days, 1 day, 3 days, 7 days and 11 days after treatment, the expression of NR2B subunit protein in the dorsal horn of spinal cord was measured by light microscope 15 days after (HWT); treatment. The expression of NR2B subunit protein in the dorsal horn of spinal cord was measured by light microscope 15 days after (HWT); treatment. Results after modeling, HWT in CS,CP group was lower than that in SS,SP group, and HWT in CP group was higher than that in CS group after treatment. Under light microscope, the diameter of axons and the thickness of myelin sheath in CP group were significantly higher than those in CS group (P 0.01. The expression of NR2B in spinal dorsal horn of rats in). CP group was lower than that in CS group (F 鈮，
本文编号：2519197
[Abstract]:Objective to observe the effect of pulse radiofrequency (PRF) on pain behavior and histology of injured sciatic nerve node in rats with neuropathic pain after chronic compression of (CCI), and to detect the expression of NR2B subunit in spinal dorsal horn. Methods 48 Sprague-Dawley rats were randomly divided into Sham-Sham (SS) group, Sham-PRF (SP) group, CCI-Sham (CS) group and CCI-PRF (CP) group. CS, CP group rats ligated the right sciatica trunk to make CCI model, SS,SP group only isolated the sciatica without ligating. On the 15th day after operation, PRF,SS,CS group only placed electrode needle without electricity in SP group. Before modeling, 3 days, 7 days, 15 days, 1 day, 3 days, 7 days and 11 days after treatment, the expression of NR2B subunit protein in the dorsal horn of spinal cord was measured by light microscope 15 days after (HWT); treatment. The expression of NR2B subunit protein in the dorsal horn of spinal cord was measured by light microscope 15 days after (HWT); treatment. Results after modeling, HWT in CS,CP group was lower than that in SS,SP group, and HWT in CP group was higher than that in CS group after treatment. Under light microscope, the diameter of axons and the thickness of myelin sheath in CP group were significantly higher than those in CS group (P 0.01. The expression of NR2B in spinal dorsal horn of rats in). CP group was lower than that in CS group (F 鈮，
本文编号：2519197
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