脾虚证动物模型的差异性研究

发布时间：2017-12-27 12:15

本文关键词：脾虚证动物模型的差异性研究　出处：《黑龙江中医药大学》2005年硕士论文　论文类型：学位论文

【摘要】：脾虚证是中医临床常见的证候之一,也是当前中医现代化研究中的一个重要内容。在脾虚证的实验研究中,脾虚证动物模型已成为决定实验成败的重要环节。近二十年,对脾虚证动物模型的探索主要侧重于对不同因素造模方法的研究,据不完全统计造模方法已近20种。但对这些模型之间差异性的研究甚少,而这种差异是客观存在的。这种现状的结果是在科研时对动物模型的选择常具有盲目性、随意性,缺乏客观的选择标准和评价,以致严重影响了科研结果的可靠性与准确性。为此,在进一步完善造模方法的同时揭示不同模型间的差异已成为提高中医药科研水平和层次的前提。本实验采用耗气破气、耗气破气加饥饱失常、劳倦过度加饥饱失常和劳倦过度加饮食失节四种方法建立脾虚动物模型,对实验动物的一般表现、行为学、生物化学、免疫学以及形态学方面进行了比较研究。实验结果显示,四组脾虚模型动物均出现脾虚证的典型表现,如体重增长缓慢,肌力减弱,外周血T淋巴细胞百分率下降,血清D-木糖含量降低或异常升高以及小肠绒毛的相应改变,四种模型造模成功。并且根据上述指标的改变评价了四种脾虚证动物模型的造模方法,进一步证实不同的造模方法在不同的指标方面确实存在差异。本实验结果初步揭示了脾虚证动物模型的共性与个性特征,为中医脾虚证的实质研究及临床新药的研制在动物模型的选择上提供了可靠的客观依据,使该方面的科学研究具有较强的针对性和目的性,进而提高科研成果的质量和水平,更易于广泛地推广应用。
[Abstract]:Spleen deficiency syndrome is one of the common syndromes in traditional Chinese medicine, and it is also an important content in the research of the modernization of traditional Chinese medicine. In the experimental study of spleen deficiency syndrome, the animal model of spleen deficiency syndrome has become an important link to determine the success or failure of the experiment. In the last twenty years, the exploration of the animal model of spleen deficiency syndrome mainly focused on the study of the modeling methods of different factors. According to the incomplete statistics method, nearly 20 kinds of models have been made. However, there are few studies on the differences between these models, and the differences are objective. The result of this situation is that the choice of animal models is often blindness and randomness in scientific research, lacking of objective selection criteria and evaluation, which seriously affects the reliability and accuracy of scientific research results. Therefore, to further improve the modeling methods and to reveal the differences between different models has become a prerequisite for improving the level and level of Chinese medicine research.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

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