以HBc颗粒为呈现载体的猪囊虫疫苗的构建及其免疫学研究

发布时间：2017-12-27 13:04

本文关键词：以HBc颗粒为呈现载体的猪囊虫疫苗的构建及其免疫学研究　出处：《中国人兽共患病杂志》2005年02期 　论文类型：期刊论文

【摘要】：目的构建以HBc为载体的带有三个猪囊虫抗原表位(n1,n2,n3)的重组融合表达质粒pET-Δc-3n,表达出融合蛋白并纯化,通过免疫小鼠研究其体液免疫效果。方法用PCR法将三个猪囊虫表位分别插入HBc序列的第78-79位之间以及149位之后,再将该序列克隆入pET28a载体中,构建重组表达质粒,用大肠杆菌BL21作宿主菌表达出融合蛋白,并命名为PCCE,纯化后免疫小鼠,检测小鼠的体液免疫应答。用绦虫卵攻击免疫小鼠,并观察疫苗的保护作用。结果测序结果表明重组质粒构建成功,SDSPAGE显示融合蛋白表达正确,并有多聚体形成现象,ELISA检测到高滴度抗体。小鼠体内绦虫卵攻击试验表明疫苗PCCE的
[Abstract]:Objective to construct a recombinant fusion expression plasmid pET- Delta c-3n with three epitopes of Cysticercus cellulosae (N1, N2, N3) based on HBc, and to express fusion protein and purify it, and to study its humoral immunity effect by immunization in mice. Methods by PCR method between three cysticercosis epitopes were inserted into the HBc sequence of 78-79 and 149, then the sequence was cloned into the pET28a vector, the recombinant expression plasmid in Escherichia coli BL21 as the host strain to express fusion protein, named PCCE, immunized mice after purification, humoral immunity the detection response in mice. The immunized mice were attacked with tapeworm eggs and the protective effect of the vaccine was observed. The results showed that the recombinant plasmid was successfully constructed. SDSPAGE showed that the expression of the fusion protein was correct, and the polypolymer was formed, and the high titer antibody was detected by ELISA. The test of Taenia egg attack in mice shows that the vaccine PCCE
【作者单位】：南京师范大学生命科学学院南京军区军事医学研究所南京军区军事医学研究所南京军区军事医学研究所南京军区军事医学研究所南京军区军事医学研究所南京军区军事医学研究所
【基金】：江苏省“十五”攻关课题
【分类号】：R392.11
【正文快照】： 囊虫病是由猪带绦虫的幼虫——猪囊尾蚴感染猪或人而引起的人畜共患寄生虫病,在我国有27个省、市自治区发现本病。目前关于囊虫病的防治还未有成熟的手段,过去的化学药物治疗以及虫体抗原免疫都已经暴露了很大的局限性。作为综合性防治措施之一,研制较为长效的寄生虫病疫苗极

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