美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响

发布时间：2017-12-27 13:39

本文关键词：美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响　出处：《中国病理生理杂志》2014年12期 　论文类型：期刊论文

【摘要】：目的:观察葡聚糖硫酸钠(DSS)诱导小鼠溃疡性结肠炎(UC)模型中辅助性T细胞(Th1、Th17亚群)及调节性T细胞(Treg)细胞亚群的变化,探讨美沙拉嗪(MSLZ)治疗UC的免疫学机制。方法:采用流式细胞分析术检测DSS诱导的小鼠UC模型结肠组织及外周血单个核细胞中白细胞介素17(IL-17)、γ-干扰素(IFN-γ)及核转录因子Foxp3的表达,并检测MSLZ预治疗对小鼠UC模型Th1、Th17和Treg亚群的影响。结果:在DSS诱导的小鼠UC模型中,其外周血单个核细胞(PBMC)中CD3+T细胞高表达IL-17、IFN-γ及Foxp3,肠黏膜单个核细胞(LPMC)中CD3+T细胞高表达IFN-γ和Foxp3,但IL-17的表达与对照组无差异。进一步发现UC模型小鼠LPMC中Th17、Th1和Treg均显著高于对照组,但PBMC中只有Treg高于对照组。MSLZ预治疗能显著下调UC模型小鼠PBMC和LPMC中Th17、Th1和Treg细胞亚群。结论:DSS诱导的小鼠UC模型中CD4+T细胞亚群Th1、Th17及Treg细胞显著升高,提示CD4+T细胞亚群在UC发病中起重要作用,美沙拉嗪可能通过调节Th1、Th17及Treg细胞亚群发挥抗炎及治疗UC作用。
[Abstract]:Objective: To observe the changes of helper T cells (Th1, Th17 subsets) and regulatory T cell (Treg) cell subsets in dextran sulfate sodium (DSS) induced ulcerative colitis (UC) model, and to explore the immunological mechanism of mesalazine (MSLZ) in the treatment of UC. Methods: using flow cytometry to detect DSS induced colon tissue of UC mice peripheral blood mononuclear cells and interleukin 17 (IL-17) and interferon gamma (IFN- gamma) and the expression of nuclear transcription factor Foxp3, and detection of MSLZ pretreatment on UC in mice models of Th1, Th17 and Treg subgroup. Results: in the DSS induced UC model, the CD3+T cells in peripheral blood mononuclear cells (PBMC) overexpressed IL-17, IFN-, gamma and Foxp3. The CD3+T cells in intestinal mucosal mononuclear cells (LPMC) highly expressed IFN- gamma and Foxp3, but the expression of CD3+T was not different from that in the control group. It was further found that Th17, Th1 and Treg in LPMC of UC model mice were significantly higher than those of the control group, but only Treg in PBMC was higher than that of the control group. MSLZ pretherapy could significantly reduce the Th17, Th1 and Treg subsets of PBMC and LPMC in UC model mice. Conclusion: the CD4+T cell subsets Th1, Th17 and Treg cells increased significantly in DSS induced UC mice, suggesting that CD4+T cell subsets play an important role in the pathogenesis of UC. Mesalazine may play an anti-inflammatory and therapeutic role by regulating Th1, Th17 and Treg cell subsets.
【作者单位】：暨南大学第一临床医学院;广东医学院检验医学研究所;广东医学院;广东省医学分子诊断重点实验室;
【基金】：国家自然科学基金资助项目(No.81273237;No.30972779) 广东省科技计划项目(No.2011B061300098)
【分类号】：R574.62;R-332
【正文快照】： 溃疡性结肠炎(ulcerative colitis,UC)是炎症性肠病的一种,是一类病因不明的以胃肠道慢性非特异性炎症为主要表现的疾病,近年来成为消化系统常见的疾病,其主要临床表现为腹痛与腹泻[1]。关于其病因目前大多数研究认为与环境、遗传、感染及免疫学因素相关,而免疫学因素被认为与

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