大鼠脊髓全横断后相邻节段脊髓神经元NT-3的表达
本文关键词：大鼠脊髓全横断后相邻节段脊髓神经元NT-3的表达 出处：《山西医科大学》2005年硕士论文 论文类型：学位论文
【摘要】：目的 研究大鼠脊髓全横断后,NT-3 在损伤部位相邻节段脊髓神经元随时间的表达变化,为进一步探索脊髓损伤后的再生修复机制和神经营养因子治疗脊髓损伤提供实验依据。方法 体重为 250 g 左右成年 SD 大鼠48 只,随机分为六组。分正常组、假手术组及胸 10 脊髓节段全横断术后 1天、 3 天、 7 天及 14 天组。动物到达存活时间后,应用免疫组织化学SABC 法和图像分析技术检测 NT-3 在脊髓损伤相邻节段腹、背角的表达和分布。结果 正常组的 NT-3 免疫反应阳性细胞主要分布在腹角的大神经元和少量小神经元及背角的神经元。损伤区上段的 NT-3 免疫反应阳性细胞数,在脊髓腹、背角的表达,均于术后 1 天组开始明显增高并达到高峰,术后 3天、7 天组逐渐回落,术后 14 天组接近正常水平。而损伤区下段的 NT-3 免疫反应阳性细胞数,在脊髓腹、背角的表达均于术后 1 天组开始明显增高,术后 3 天组达到高峰,于 7 天组开始下降,到 14 天组接近正常水平。结论在脊髓全横断术后,脊髓神经元对 NT-3 的需求增加,提示 NT-3 在脊髓修复中起重要作用。
[Abstract]:Objective to investigate the expression of NT-3 in adjacent spinal cord neurons at different time points after spinal cord transection, and to provide experimental evidence for further exploration of regeneration and repair mechanism after spinal cord injury and neurotrophic factor in spinal cord injury. Methods a total of 48 adult SD rats with a weight of 250 g were randomly divided into six groups. It was divided into 1, 3, 7 and 14 days after total transection in the normal group, the sham operation group and the thoracic 10 segment of the spinal cord. After the animals reached their survival time, the expression and distribution of NT-3 in the abdominal and dorsal segments of spinal cord adjacent to spinal cord injury were detected by immunohistochemical SABC and image analysis techniques. Results the NT-3 immunoreactive cells in the normal group were mainly distributed in the large neurons in the ventral angle and a small amount of neurons in the small neurons and the dorsal horn. The number of NT-3 immunoreactive cells in the upper part of the injured area in the ventral and dorsal horn of the spinal cord increased significantly and reached the peak on the 1 day after operation. After 3 days and 7 days, the group gradually decreased, and the group was close to the normal level on the 14 day after operation. The number of NT-3 immunoreactive cells in the spinal cord at the lower part of the injured area increased significantly on the 1 day after operation, and reached the peak on the 3 day after operation. It began to decline in the 7 day group, and it was close to the normal level in the 14 day group. Conclusion after full transection of spinal cord, the demand for NT-3 is increased in spinal cord neurons, suggesting that NT-3 plays an important role in the repair of spinal cord.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329
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