心肌缺血对大鼠延髓利钠肽受体表达的影响

发布时间：2017-12-27 22:16

本文关键词：心肌缺血对大鼠延髓利钠肽受体表达的影响　出处：《中国病理生理杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的:探讨大鼠延髓腹侧部利钠肽受体A(NPR-A)、利钠肽受体C(NPR-C)、胆碱乙酰转移酶(ChAT)和酪氨酸羟化酶(TH)的表达在心肌缺血后不同时程的动态变化。方法:大鼠分为空白对照组、假手术组和模型组。建立心肌缺血模型后,于术后3、7、14、18和28 d取材,用Western blotting方法检测延髓腹侧部吻段、尾段内NPR-A、NPR-C、ChAT和TH表达。结果:在模型复制后的上述时点,延髓腹侧部NPR-A的表达显著升高(P0.05),NPR-C表达的升高晚于NPR-A,TH的表达显著低于空白对照组(P0.05),在延髓腹侧部吻段ChAT的表达低于空白对照组(P0.05),而在尾段与对照组无显著差异。结论:延髓心血管中枢内NPR-A和NPR-C的表达在心肌缺血后均显著升高,而ChAT和TH的表达则显著降低。利钠肽、ChAT和TH可能都参与了心肌缺血后心血管系统的植物神经调控。
[Abstract]:Objective: To investigate the dynamic changes of the expression of natriuretic peptide receptor A (NPR-A), natriuretic peptide receptor C (NPR-C), choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) in the ventral side of the medulla oblongata in different time periods after myocardial ischemia. Methods: the rats were divided into the blank control group, the sham operation group and the model group. After establishing the myocardial ischemia model, after 3, 7, 14, 18 and 28 d after operation, the expression of NPR-A, NPR-C, ChAT and TH in the ventral part of the medulla oblongata were detected by Western blotting. Results: in the above point model after replication, expression of ventral medulla NPR-A increased significantly (P0.05), the expression of NPR-C increased after NPR-A, the expression of TH was significantly lower than the control group (P0.05), the expression in the ventral medulla of kiss ChAT lower than the control group (P0.05), and at the end of the period no significant difference from the control group. Conclusion: the expression of NPR-A and NPR-C in the cardiovascular center of the medulla significantly increased after myocardial ischemia, while the expression of ChAT and TH decreased significantly. Natriuretic peptide, ChAT and TH may be involved in the regulation of the autonomic nervous system in the cardiovascular system after myocardial ischemia.
【作者单位】：河北省疾病预防控制中心医学研究所;唐山职业技术学院基础医学部解剖学教研室;河北师范大学生命科学学院生理学教研室;
【基金】：河北省卫生厅医学科学研究重点课题(No.20090044)
【分类号】：R363
【正文快照】： 利钠肽受体(natriuretic peptide receptors,NPRs)主要包括NPR-A、NPR-B和NPR-C,其中NPR-A和NPR-B属于具有鸟苷酸环化酶活性的受体,而NPR-C通常被认为是可以从循环中清除利钠肽的受体。利钠肽通过激活细胞膜上的NPR-A或NPR-B参与对抗交感神经系统和肾素-血管紧张素系统的促进

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