豚鼠气单胞菌单克隆抗体及抗独特型单克隆抗体的制备研究

发布时间：2017-12-28 00:09

本文关键词：豚鼠气单胞菌单克隆抗体及抗独特型单克隆抗体的制备研究　出处：《第四军医大学》2006年硕士论文　论文类型：学位论文

【摘要】：豚鼠气单胞菌(Aeromonas caviae)是养殖及野生鱼、虾、蛙、甲鱼等生物常见的细菌性病原体，可通过受损伤的皮肤及伤口感染上述生物，，并可进一步通过食物链感染人。此菌所致鱼类等豚鼠气单胞菌病具有流行广、发病快、死亡率高等特点，常常造成养殖渔业的巨大经济损失，严重危害渔业及其它水产业的发展。目前国内外有关豚鼠气单胞菌鱼病的研究多局限于病原微生物学方面，对该病的诊断主要依靠临诊病理观察及细菌学鉴定，两种方法在准确性及检测速度上均有一定限制；防治方面主要利用抗生素或减活、灭活疫苗，安全性和有效性均不理想。鉴于此，本研究设计制备抗豚鼠气单胞菌单克隆抗体并进而制备其独特型单克隆抗体，为豚鼠气单胞菌病的检测及防治提供新的技术方法，并为其它鱼用疫苗的研制提供参考。具体实验方法及结果如下： (1) 通过动物免疫、细胞融合、筛选、克隆化及利用ELISA检测技术，制备并鉴定抗豚鼠气单胞菌的单克隆抗体(mAb)。共得到7株能稳定分泌mAb的细胞株，分别命名为：0E10、1A2、1C4、1F1、1F10、1H8和2A1。染色体鉴定结果7株细胞染色体均符合杂交瘤细胞染色
[Abstract]:Aeromonas guinea pig (Aeromonas caviae) is a common bacterial pathogen of aquaculture and wild fish, shrimp, frog, turtle and other organisms. It can infect the above organisms through the injured skin and wound, and can further infect people through the food chain. Aeromonas aeruginosa, such as fishes and other guinea pigs, is characterized by widespread epidemic, fast onset and high mortality. It often causes huge economic losses in aquaculture, and seriously endangering the development of fishery and other aquatic industries. At present, the domestic and foreign research on Aeromonas caviae disease confined to the microbiology, diagnosis of the disease mainly depends on clinical pathology and bacteriology, two kinds of methods in both accuracy and speed of detection is limited; prevention mainly using antibiotics or live attenuated and inactivated vaccine, safety and the effectiveness is not ideal. In view of this, we designed and prepared monoclonal antibodies against Aeromonas guinea pigs, and then prepared their unique monoclonal antibodies, providing new technical methods for the detection and prevention of Aeromonas guinea pigs, and providing references for the development of other fish vaccines. The specific experimental methods and results are as follows:
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

【参考文献】