寇氏隐甲藻突变株的糖、氮代谢对产油的影响

发布时间：2017-12-28 05:21

本文关键词：寇氏隐甲藻突变株的糖、氮代谢对产油的影响　出处：《中国粮油学报》2014年11期 　论文类型：期刊论文

更多相关文章： 寇氏隐甲藻突变株 碳氮代谢 产油 DHA

【摘要】：为了探索在寇氏隐甲藻(crypthecodinium cohnii)突变株的营养生长和油脂积累过程中对碳源和氮源的需求及其影响。通过对照组和5个不同的补糖试验组进行研究,各组经发酵7 d收获,并测定生物量、含油量、DHA含量和DHA产量。结果显示各组在发酵培养24 h时,碳消耗量约为22.5%,氮耗约为50%。随着不同时间的补糖,测定碳耗和氮耗虽有小幅度升降,但在144~168 h时碳耗、氮耗和碳/氮比基本稳定,各组间差异较小。研究表明,前72 h是寇氏隐甲藻生长的重要时期,当碳/氮比在15∶1左右时补糖最佳;当氮源耗尽时补糖不仅可以促进油脂的产生,而且对DHA的形成也是有利的。
[Abstract]:In order to explore in C. cohnii (Crypthecodinium cohnii) and its influence on the demand of carbon source and nitrogen source nutrition mutant growth and lipid accumulation in plant. Through the control group and 5 different sugar supplement test groups, each group was harvested by 7 d fermentation, and the biomass, oil content, DHA content and DHA yield were measured. The results showed that the carbon consumption of 24 h was about 22.5% and the nitrogen consumption was about 50%. With the increase of sugar at different times, the carbon consumption and nitrogen consumption had a slight rise and fall. However, at 144~168 h, the carbon consumption, nitrogen consumption and C / N ratio were basically stable. The research shows that the first 72 h is an important period of growth of C. cohnii, when the carbon / nitrogen ratio in 15 to about 1 of the best sugar supplement; when nitrogen depletion when sugar supplement can not only promote the oil production, but also for the formation of DHA is beneficial.
【作者单位】：武汉轻工大学食品科学学院;中国科学院武汉病毒研究所;武汉博特尔油脂科技有限公司;
【基金】：“十二五”国家科技支撑计划(2011BAD02B00)
【分类号】：R383
【正文快照】： 寇氏隐甲藻(Crypthecodinium cohnii)是一种海生、无色、异养双鞭毛甲藻,多生长在腐败的大型褐藻上,也可在琼脂板上生长,属于从原核生物向真核生物的过度类型。目前国内外文献报道寇氏隐甲藻藻体中,产油率高达10%~15%,DHA质量分数高达35%~40%。2010国家卫生部正式批准由寇氏隐

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