造血调控因子HAPO、PF4对造血干细胞调节的体内外研究

发布时间：2017-12-28 13:08

本文关键词：造血调控因子HAPO、PF4对造血干细胞调节的体内外研究　出处：《中国协和医科大学》2005年博士论文　论文类型：学位论文

【摘要】：目的研究人促血液血管细胞生成素(Hemangiopoietin，HAPO)对CD133~+造血干细胞的作用，探讨其生物学功能。方法采用细胞液体培养、半固体培养、免疫荧光标记流式细胞仪测定、显微镜观察照相等方法。结果 1．在加SCF、TPO、IL-3的无血清无基质培养体系中，培养的人骨髓CD133~+细胞，，7天时流式细胞仪测定对照组CD133~+细胞含量为41.92％，HAPO组CD133~+细胞含量为56.70％；HAPO组中CD133~+细胞数由原来的6.8×10~4增加为(2.88±0.12)×10~6，细胞数扩增了41.98倍；对照组中CD133~+细胞数由原来的6.8×10~4增加为(1.79±0.02)×10~6，细胞数扩增了26.09倍；10天时流式细胞仪测定对照组CD133~+细胞含量为32.60％，HAPO组CD133~+细胞含量为49.02％；HAPO组中CD133~+细胞数扩增了356倍，而对照组中CD133~+细胞数扩增了98倍。2．扩增7天时，流式细胞仪测定对照组细胞粘附分子CD31细胞含量对照组为68.27％，HAPO组CD31细胞含量为76.89％；细胞粘附分子CD49d表达对照组80.11％、HAPO组CD49d细胞含量为88.17％。3．将5×10~3个CD133~+细胞接种到1ml甲基纤维素半固体集落培养基中，PBS对照组生成GM-CFU 134.5±9.2个，HAPO组生成GM-CFU 167.5±4.5个，
[Abstract]:Objective to study the effect of human Hemangiopoietin (HAPO) on CD133~+ hematopoietic stem cells and to explore its biological function. Methods the methods of cell culture, semisolid culture, immunofluorescent labeling flow cytometer and microscope observation were used. Results 1. in SCF, TPO, IL-3 and serum-free medium culture system, human bone marrow CD133~+ cells cultured for 7 days, when the flow cytometry content of CD133~+ cells control group 41.92%, HAPO group of CD133~+ cells was 56.70%; the number of CD133~+ cells in the HAPO group from the original 4 increased to 6.8 * 10~ (2.88 + 0.12) * 10~6, the cell number was 41.98 times; the control group: the number of CD133~+ cells from 6.8 * 10~4 to increase (1.79 + 0.02) * 10~6, the cell number was 26.09 times; 10 days were determined by flow cytometry in CD133~+ cells of control group was 32.60%, HAPO group of CD133~+ cells the content is 49.02%; the number of CD133~+ cells in HAPO group was 356 times, while the control group in the number of CD133~+ cells was 98 times. 2. after 7 days of amplification, flow cytometry was used to determine the cell adhesion molecule CD31 cell content in the control group, 68.27% in the control group, and 76.89% in the HAPO group, while the CD49d expression in the cell adhesion molecule was 80.11% in the control group and 88.17% in the HAPO group, and the CD49d cell content in the CD31 group was 88.17%. 3., 5 x 10~3 CD133~+ cells were inoculated into 1ml semi-solid medium, and PBS control group generated GM-CFU 134.5 + 9.2, HAPO group generated GM-CFU 167.5 + 4.5.
【学位授予单位】：中国协和医科大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R329

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