2011年济南市某污水河中肠道病毒的分离鉴定与序列分析

发布时间：2017-12-30 19:46

本文关键词：2011年济南市某污水河中肠道病毒的分离鉴定与序列分析　出处：《山东大学学报(医学版)》2014年11期 　论文类型：期刊论文

【摘要】：目的监测济南市区地表河水中的人类肠道病毒(HEV),分析其基因特征。方法于2011年5、6、7月份对流经济南市中心城区的西泺河各采集1份河水标本,经过阴离子膜吸附的方法浓缩后,接种RD、HEp-2和L20b细胞进行病毒分离。测定分离株VP1编码区序列并进行序列分析。结果共分离到HEV 9株,其中埃可病毒6型(Echovirus 6,E6)4株,柯萨奇B2病毒(Coxsackievirus B2,CVB2)5株。VP1核苷酸序列分析显示,4株E6病毒与2010年山东省临沂市脑膜炎病例分离株的同源性较高(95.9%~96.7%),提示当地有发生E6引起的脑膜炎病例的可能性;与济南市2010年污水分离株的同源性为94.5%~99.7%。5株CVB2病毒之间有较高的核苷酸同源性(98.4%~100%),与往年山东省急性弛缓性麻痹病例分离株之间同源性为80.9%~95.2%。结论生活污水的排入河可作为HEV环境监测的采样点,我国无专门HEV病例监测系统,环境监测是了解一个地区HEV流行的重要途径。
[Abstract]:Objective to monitor the Ji'nan urban surface water in human enterovirus (HEV), to analyze the genetic characteristics. In 2011 5,6,7 month economic downtown West South convection Luohe River each collected 1 specimens, through the anion exchange membrane adsorption concentration method after inoculation with RD, HEp-2 and L20b cells were isolated from determination of virus isolation. The sequence of VP1 encoding region and sequenced. The results were isolated from 9 strains of HEV, including echovirus 6 (Echovirus 6, E6) and 4 strains of Coxsackie virus B2 (Coxsackievirus B2 CVB2).VP1 nucleotide sequence analysis showed that 5 strains of meningitis, 4 isolates of E6 virus and Shandong city of Linyi province in 2010 high homology (95.9%~96.7%), suggest the possibility of local meningitis cases caused by E6; and in 2010 Ji'nan city sewage isolates have high homology to 94.5%~99.7%.5 strain of CVB2 virus nucleotide homology (98.4%~1 00%), homologous with previous acute flaccid paralysis cases in Shandong province between isolates of 80.9%~95.2%. conclusion sewage discharged into the river can be used as the sampling points HEV environmental monitoring, special HEV surveillance system in China, environmental monitoring is an important way to understand the prevalence of HEV in a region size.

【作者单位】：山东省传染病预防控制重点实验室山东省疾病预防控制中心;济宁医学院;《中华行为医学与脑科学杂志》编辑部;
【基金】：国家自然科学基金青年基金(81302481) 全球健康-盖茨基金(OPP1039272)
【分类号】：R373
【正文快照】： 人类肠道病毒(human enterovirus,HEV)有100多个型别(血清型),可以引起多种疾病,如急性弛缓性麻痹(acute flaccid paralysis,AFP)、手足口病(hand,foot and mouth disease,HFMD)、无菌性脑膜炎(aseptic meningitis,AM)、脑炎、心肌炎、急性出血性结膜炎、类感冒病症等,其感染

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