PSGL-1基因敲除小鼠血中MIP-1γ和TNF-α的表达

发布时间：2017-12-30 18:41

本文关键词：PSGL-1基因敲除小鼠血中MIP-1γ和TNF-α的表达　出处：《中国比较医学杂志》2014年02期 　论文类型：期刊论文

更多相关文章： PSGL- TNF-α MIP-γ 基因工程动物

【摘要】：目的研究PSGL-1缺失对遗传基因工程小鼠外周血血常规的影响,并检测外周血中炎症因子IGFBP-6、TNF-α和MIP-1γmRNA表达水平。方法用血常规检测方法检测正常C57/BL/6小鼠和PSGL-1基因缺失的基因工程小鼠(PSGL-1-/-小鼠)的外周血中血常规的差异;其次,提取两种鼠的血液的mRNA,逆转录为cDNA,采用real-time PCR方法,检测C57小鼠与PSGL-1-/-小鼠外周血中炎症因子TNF-α和MIP-1γmRNA的差异。结果与对照组C57小鼠相比,PSGL-1-/-基因工程小鼠在12周龄时外周血中中性粒细胞(*P0.05)、淋巴细胞(**P0.01)和白细胞细胞(***P0.001)总数显著增加炎症因子TNF-α以及MIP-1γ表达增加(P0.05)。结论 PSGL-1的缺失改变了小鼠细胞因子并影响了外周血的血细胞组成,MIP-1γ和TNF-α炎症因子上调,有可能影响了小鼠的免疫功能。
[Abstract]:Objective to study the effect of PSGL-1 deletion on peripheral blood routine in genetically engineered mice and to detect the inflammatory factor IGFBP-6 in peripheral blood. The expression levels of TNF- 伪 and MIP-1 纬 mRNA. Methods normal C57 / BL / 6 mice and genetically engineered mice with PSGL-1 gene deletion were detected by blood routine method (TNF- 伪 and MIP-1 纬). The difference of blood routine in peripheral blood of PSGL-1-r-mice; Secondly, the mRNAs were extracted from the blood of two kinds of mice, and reverse transcripted to cDNA. the method of real-time PCR was used. The levels of inflammatory cytokines TNF- 伪 and MIP-1 纬 mRNA in peripheral blood of C57 mice and PSGL-1-r / -mice were detected, and the results were compared with those of control C57 mice. PSGL-1-r- genetically engineered mice were found to have neutrophils in peripheral blood at 12 weeks of age (P0.05). The total number of TNF- 伪 and MIP-1 纬 increased significantly (P 0.05). Conclusion the deletion of PSGL-1 changes the cytokines of mice and affects the blood cell composition of peripheral blood. The upregulation of MIP-1 纬 and TNF- 伪 inflammatory factors may affect the immune function of mice.
【作者单位】：广东药学院血管生物学研究所;
【基金】：国家自然基金(31271455) 广东省医学科研基金(A2013312)
【分类号】：R363
【正文快照】： P-选凝素糖蛋白配体1(P-selectin glycoproteinligand 1,PSGL-1)是20世纪90年代初期发现的一种黏附分子,具有同源二聚体结构的跨膜糖蛋白,表达于白细胞的膜表面。PSGL-1是P-选凝素的配体,同时也是L-选凝素和E-选凝素的配体,研究表明其在炎症反应中发挥重要作用。在炎症环境中,

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