强迫游泳应激对大鼠甲状腺激素及垂体促甲状腺激素β亚基表达水平的影响

发布时间：2017-12-30 23:01

本文关键词：强迫游泳应激对大鼠甲状腺激素及垂体促甲状腺激素β亚基表达水平的影响　出处：《中国医科大学学报》2014年12期 　论文类型：期刊论文

【摘要】：目的探讨强迫游泳应激对Wistar大鼠甲状腺功能及垂体促甲状腺激素(TSH)β亚基(TSHβ)蛋白和m RNA表达水平的影响。方法选择6周龄Wistar雄性大鼠随机分成5组,除对照组外,强迫游泳10 min后分别于10 min、2 h、12 h及24 h断头,取血,化学发光分析法测定各组大鼠血清TSH、游离三碘甲腺原氨酸(FT3)和游离甲状腺素(FT4)水平;留取各组Wistar大鼠垂体,应用免疫组织化学染色检测TSHβ蛋白表达水平,垂体组织匀浆应用实时定量PCR分析TSHβm RNA表达水平。结果强迫游泳后2 h组、12 h组和24 h组血清FT3、FT4均升高,较对照组均无统计学差异(P0.05),10 min组、2 h组及24 h组血清TSH水平较对照组无明显变化,但12 h组较其余各组均明显升高(P0.05)。垂体TSHβ蛋白及m RNA表达强迫游泳后12 h组也较其他组明显升高(P0.05)。结论强迫游泳应激可影响Wistar大鼠甲状腺功能,使血中甲状腺素水平升高。强迫游泳应激后Wistar大鼠垂体TSHβ蛋白及m RNA表达水平增加,峰值出现在12 h。提示丘脑-垂体-甲状腺轴可能在急性心理和生理应激时同样发挥重要的调节作用。
[Abstract]:Objective to investigate the effects of forced swimming stress on thyroid function and pituitary thyrotropin (TSH) 尾 subunit TSH 尾 in Wistar rats. Methods the 6-week-old male Wistar rats were randomly divided into 5 groups. In addition to the control group, after forced swimming for 10 min, the head was cut off for 12 h and 24 h, respectively. The blood samples were taken and the serum TSH was determined by chemiluminescence analysis. Free triiodothyronine (FT3) and free thyroxine (FT4); The pituitary gland of each group of Wistar rats was retained and the expression of TSH 尾 protein was detected by immunohistochemical staining. The expression of TSH 尾 m RNA in pituitary homogenate was analyzed by real-time quantitative PCR. Compared with the control group, there was no significant difference in serum TSH levels between the control group and the control group. There was no significant change in the level of serum TSH between the two groups and the 24 hour group. The expression of TSH 尾 protein and m RNA in pituitary gland was significantly higher in 12h group than in other groups. The expression of TSH 尾 protein and m RNA in 12 h group was significantly higher than that in other groups (P 0.05). Conclusion forced swimming stress can affect thyroid function in Wistar rats. The expression of TSH 尾 protein and m RNA in pituitary of Wistar rats was increased after forced swimming. The peak appeared at 12 h, suggesting that the thalamus-pituitary-thyroid axis may also play an important regulatory role in acute psychological and physiological stress.
【作者单位】：中国医科大学附属第一医院内分泌科;中国医科大学保健科;
【基金】：辽宁省高等学校创新团队项目(LT2012015)
【分类号】：R335.2
【正文快照】： 甲状腺素不仅在细胞的代谢、氧消耗,组织的生长、成熟和分化以及人体脂肪、碳水化合物代谢调节等方面起重要作用[1],下丘脑-垂体-甲状腺轴(hypothalamus-pituitary-thyroid,HPT)也构成了人体应激反应系统的重要一环[2,3]。随着神经内分泌调控及其机制研究的进展,应激对机体的

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