点拨腰大肌干预椎间盘损伤大鼠模型的实验研究

发布时间：2017-12-30 23:34

本文关键词：点拨腰大肌干预椎间盘损伤大鼠模型的实验研究　出处：《中国中医骨伤科杂志》2014年12期 　论文类型：期刊论文

【摘要】：目的:通过前路针刺损伤椎间盘建立腰椎间盘损伤大鼠模型,观察该模型是否伴有腰大肌被动拉伸张力变化以及点拨腰大肌对模型的影响。方法:将Wistar雄性大鼠随机分为假手术组、模型组与治疗组,通过前路手术损伤椎间盘造模。治疗组行点拨腰大肌干预,隔日1次,共10次。观察大鼠行为学、腰椎间盘形态学、IL-1β阳性表达以及腰大肌被动拉伸张力的变化。结果:模型组与假手术组相比,垂直方向活动度减少(P0.05),椎间盘纤维环、髓核损伤明显,椎间盘IL-1β免疫组化阳性表达积分光密度增高(P0.01),腰大肌弹性模量降低(P0.01);治疗组与模型组比较,椎间盘纤维环及髓核病理变化有所好转,IL-1β免疫组化阳性表达积分光密度降低(P0.01),腰大肌弹性模量有所提高。结论:前路椎间盘损伤大鼠模型的腰大肌弹性模量有所降低,点拨腰大肌可使其有所恢复,同时可有效改善大鼠椎间盘形态,降低炎性因子IL-1β的表达。
[Abstract]:Objective: through anterior acupuncture injury of intervertebral disc to establish lumbar disc injury model rats, observe whether the model effects by psoas muscle tension and change the passive direction of the psoas muscle model. Methods: Wistar male rats were randomly divided into sham operation group, model group and treatment group, the anterior surgery injury the intervertebral disc model. The treatment group underwent coaching psoas major intervention, 1 every other day for 10 times. To observe the behavior of rats, lumbar disc morphology, IL-1 beta expression and change of psoas muscle tension passive. Results: the model group compared with the sham operation group, the vertical activity reduced (P0.05), annulus fibrosus and nucleus pulposus of intervertebral disc injury obviously, IL-1 P immunohistochemical expression of integral optical density (P0.01) increased, reducing the psoas muscle elastic modulus (P0.01); the treatment group compared with the model group, the pathological changes of nucleus pulposus of intervertebral disc and ring fiber has improved, IL-1 Beta immunohistochemical expression of integral optical density (P0.01) decreased, psoas muscle elastic modulus increased. Conclusion: anterior disc psoas muscle injury rat model of elastic modulus decreased, indicated the psoas muscle can make the recovery, which can effectively improve the rat intervertebral disc shape, decrease the expression of inflammation factor IL-1 beta.

【作者单位】：北京中医药大学针灸推拿学院;北京市海淀区中医医院;北京按摩医院;
【基金】：北京中医药大学校级自主选题项目(No.2011-JYBZZ-JS068)
【分类号】：R-332;R681.5
【正文快照】： 椎间盘源性腰痛的发病率占腰痛患者总发病率的40%,指起源于椎间盘本身的疼痛[1,2]。其典型症状为腰腹痛、腰臀痛、腰痛连及腹股沟部广泛性疼痛,与中医所描述的“带脉瘀滞型腰痛”的典型表现。“腹腰脊痛,冲阴股”很相仿[3~5]。临床触诊发现,除棘突间压痛外,还存在髂腰肌,尤其

【参考文献】