MicroRNA-1抑制内皮素-1表达机制的初步研究

发布时间：2017-12-31 11:43

本文关键词：MicroRNA-1抑制内皮素-1表达机制的初步研究　出处：《第二军医大学》2007年硕士论文　论文类型：学位论文

更多相关文章： 内皮素-1 microRNA-1 基因表达调控

【摘要】： 内皮素-1(endothelin-1 , ET-1)是目前已知最强烈的血管收缩物质之一,主要分布于心血管系统,参与了心脏发育、心肌肥厚和心律失常等心脏生理和病理生理过程。ET-1基因受不同水平的表达调控,转录后水平的调控对于ET-1基因表达具有重要意义。MicroRNA是近年来在生物体内发现的一类长度在19-22核苷酸内源性单链小分子RNA,它通过与靶基因mRNA 3’非翻译区(3’UTR)相应靶序列碱基之间不完全或完全的互补结合,抑制mRNA的翻译或引起mRNA的降解,在转录后水平发挥对靶基因的沉默效应。我们通过对MicroRNA-1和ET-1mRNA时空表达谱检测和生物信息学预测分析,发现了MicroRNA-1可能参与调控ET-1基因表达的多项线索;利用报告基因、预测靶位点序列缺失和突变等功能研究方法,证实了MicroRNA-1能够通过ET-1 3′UTR上特定靶序列在转录后水平抑制内源性ET-1基因表达。我们的研究首次发现MicroRNA对心血管活性物质的调控作用,为阐明MicroRNA在心血管生理活动和疾病中的作用提供了新的依据。
[Abstract]:Endothelin-1 endothelin-1 (ET-1) is one of the most powerful vasoconstrictors known at present, mainly distributed in the cardiovascular system, involved in cardiac development. Cardiac physiological and pathophysiological processes such as cardiac hypertrophy and arrhythmia. ET-1 gene is regulated by different levels of expression. The regulation of posttranscriptional level plays an important role in the expression of ET-1 gene. MicroRNAs are a class of endogenous single-stranded RNA with the length of 19-22 nucleotides found in vivo in recent years. It inhibits the translation of mRNA or induces the degradation of mRNA by the incomplete or complete complementary binding between the corresponding target bases of the target gene mRNA 3'untranslated region (mRNA 3'untranslated region). At post-transcriptional level, we play a silencing effect on target genes. We detect spatiotemporal expression profiles of MicroRNA-1 and ET-1mRNA and predict bioinformatics. It was found that MicroRNA-1 might be involved in the regulation of ET-1 gene expression. Using the reporter gene to predict the deletion and mutation of target sequences and other functional research methods. Confirmed that MicroRNA-1 can pass through ET-1. 3The specific target sequence of MicroRNA suppresses the expression of endogenous ET-1 gene at post-transcriptional level. Our study first discovered the regulatory effect of MicroRNA on cardiovascular active substances. It provides a new basis for elucidating the role of MicroRNA in cardiovascular physiological activities and diseases.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R341

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