小鼠吸入性鼠疫模型的组织病理学和鼠疫耶尔森氏菌重要毒力相关基因体内转录水平的研究

发布时间：2017-12-31 22:09

本文关键词：小鼠吸入性鼠疫模型的组织病理学和鼠疫耶尔森氏菌重要毒力相关基因体内转录水平的研究　出处：《吉林大学》2006年博士论文　论文类型：学位论文

【摘要】：鼠疫耶尔森氏菌(Yersina pestis)是烈性传染病鼠疫的病原体。鼠疫曾给人类带来了巨大的灾难,而且仍是21世纪的瘟疫。为了揭示吸入性鼠疫的发生、发展过程,探讨鼠疫菌致病机制,本研究建立了小鼠吸入性鼠疫模型,组织病理切片、光学及电子显微镜观察结果显示,鼠疫菌吸入后在小鼠可诱导产生肺鼠疫型和非肺鼠疫型鼠疫发生;肺鼠疫的最基本的病理改变为出血、炎细胞浸润、肺组织坏死、细菌大量增殖及晚期多脏器损伤;生存环境可能导致鼠疫菌形态及超微结构的改变,肺泡腔中菌细胞的表面被覆菌毛和F1抗原,肺间质中,在高电子密度的菌体周围可见均匀、致密较低电子密度的荚膜;在感染的过程中,除巨噬细胞和中性粒细胞外,淋巴系统也受到严重的损伤,感染晚期脾、胸腺淋巴细胞发生坏死和凋亡;免疫组化染色结果提示,鼠疫菌5种毒力相关蛋白,F1、LcrV、LcrG、Pla、pH6抗原在宿主体内均表现为菌体表面可检测到的表达;实时定量RT-PCR结果显示,其相应毒力基因在感染的不同时间及组织中呈差异性表达,这种表达的差异性体现了鼠疫病程发展过程中鼠疫菌与宿主之间的相互作用,在鼠疫菌致病机制中具有重要意义。本研究为鼠疫菌基因组学研究奠定了理论和实践基础,对鼠疫预防、治疗及致病机制研究具有重要意义。
[Abstract]:The plague, Jerson Prand (Yersina pestis), is the pathogen of the plague of the strong infectious disease. The plague had brought great disaster to mankind, and was still the plague in twenty-first Century.
In order to reveal the inhalation plague occurrence, development process, to explore the pathogenic mechanism of Yersinia pestis, this study established a mouse model of inhalation plague, histopathology, optical and electron microscopy showed that Yersinia pestis after inhalation in mice can induce lung plague and pneumonic plague; plague the most basic the pathological changes of pulmonary hemorrhage, inflammatory cell infiltration, tissue necrosis, bacterial proliferation and late multiple organ injury; living environment may cause the plague bacteria morphology and ultrastructure changes of surface coated fimbriae and F1 antigen in bacterial cells of alveoli, interstitial lung, around the high electron density was visible uniform. Compact low electron density capsule; during the course of infection in macrophages and neutrophils, the lymphatic system also suffered serious damage, infection of advanced spleen, lymphocyte necrosis and thymus Apoptosis; immunohistochemistry staining showed that related proteins of Yersinia pestis virulence, 5 F1, LcrV, LcrG, Pla, pH6 antigen in the host cell surface expression were detected; real-time quantitative RT-PCR results showed that the different time and the corresponding virulence genes in infection showed differential expression this expression, the difference reflects the interaction between Yersinia pestis and host of plague in the course of the development process, plays an important role in the pathogenesis of Yersinia pestis.
This study has laid a theoretical and practical basis for the genomics of Yersinia pestis, and is of great significance to the prevention, treatment and pathogenesis of plague.

【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R363

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