动物实验性弦脉教学模型的建立及其评价

发布时间：2018-01-02 10:44

本文关键词：动物实验性弦脉教学模型的建立及其评价　出处：《北京中医药大学》2005年硕士论文　论文类型：学位论文

【摘要】：1 目的本实验通过对家兔疼痛刺激和对家兔、犬静脉滴注去甲肾上腺素复制动物弦脉模型,使用 NX-3 型多功能脉图仪记录压力脉图,对脉图时域参数进行分析并进行脉象的属性归类,为中医脉诊教学,也为今后新药研发中加入中医脉诊指标作初步的探索。 2 方法 2.1 关于电学压力值与实际压力值换算的脉图仪压力定标实验:NX—3 多功能脉图仪黑色探头 1 和黑色探头 2 上放置 1g、2g、5g、10g、20g、50g、100g、140g砝码,NX—3 多功能脉图仪紫色探头放置 1g、2g、5g、10g、20g、50g 砝码分别记录两种脉图仪共三个探头其空载、探头上放置砝码时脉象采集仪上所显示的取脉压力值,验证砝码重与取脉压力值的线性关系。 2.2 给予家兔疼痛性刺激建立弦脉模型在非麻醉状态下将家兔行颈部手术,暴露颈动脉,经两名有经验的中医师双盲取脉,使用脉图仪记录颈动脉脉图,待家兔呼吸平稳后再由两名有经验的中医师双盲取脉,确认为平脉后在同样的取脉压力下记录颈动脉脉图,对脉图参数进行分类后做时域分析。 2.3 给予家兔、犬静脉滴注去甲肾上腺素建立弦脉模型将家兔、犬行颈部手术,暴露颈动脉, 待呼吸平稳后由两名有经验的中医师双盲取脉,确认为平脉后使用脉图仪记录颈动脉脉图,再静脉滴注去甲肾上腺素,再由两名有经验的中医师双盲取脉,确认为弦脉后在同样的取脉压力下记录家兔颈动脉脉图,犬的颈、肘和股动脉脉图并,对脉图参数进行分类后做时域分析。 3 结果 3.1 NX—3 多功能仪黑色探头 1 砝码重与电学压力值之间 R 值为 0.9993;NX—3 型多功能脉图仪黑探头 2 砝码重与电学压力值之间 R 值为 0.9997 ,NX—3 型多功能脉图仪紫探头砝码重与电学压力值之间 R 值为 0.9999。三者具有良好的线性关系。 3.2 家兔、犬教学用切诊部位 3.2.1 家兔膝关节上 8.92±0.12cm 股动脉搏动处为关脉 3.2.2 犬股动脉与股骨交叉处为关脉 3.3 家兔、犬弦股动脉指下感觉 3.3.1 家兔:①平脉指感:轻取即得,中取有力,重取反弱;一息 17～18 至;脉来和缓有力②弦脉指感:一息 14～15 至,脉位较浅,脉形长,脉势看紧张度较高,给人以“如按琴弦”的感觉。疼痛型弦脉时间可持续 30.07±4.06 分钟去甲肾上腺素型弦脉在持续滴注过程中一直存在。。 3.3.2 犬:①平脉一息 8～9;轻取既得,中取有力,重取反弱;脉来平和有力;②弦脉切脉时指下感觉与家兔弦脉大致相似,一息 7～8 至,脉位浅,脉形长,搏动比家兔甚至人的脉象更有力,脉紧张度高,有“端直以长,如按
[Abstract]:1 purposes
Through the experiments on rabbits and dogs pain stimulation on rabbits, intravenous infusion of noradrenaline to replicate the animal model of type NX-3 string pulse, multifunctional sphygmograph recording pressure pulse graph and attribute analysis of the pulse time domain parameters and pulse classification for TCM teaching, also makes a preliminary study for Chinese medicine research and development of new drugs in the future. The pulse index
2 method
2.1 on the electrical pressure pressure pulse conversion instrument calibration experiment and actual pressure: NX - more than 3 functional sphygmograph black and black 1 probe probe 2 is placed on the 1g, 2G, 5g, 10g, 20g, 50g, 100g, 140g weight, NX more than 3 functional sphygmograph purple probe placed 1g. 2G, 5g, 10g, 20g, 50g were recorded in two Farmar sphygmograph of three probes were placed on the no-load, probe weights pulse acquisition instrument shown on the pressure value, and verification of weight values of the linear relationship between the pulse pressure.
2.2 rabbits given pain stimulation model under anesthesia to establish wiry rabbits underwent neck surgery in the non exposed carotid artery by two experienced doctors with double blind pulse taking, using pulse recorded by carotid pulse, the rabbits breathing smooth again by two experienced doctors with double blind take the pulse, identified as pulse under the same pressure record carotid pulse, time domain analysis to do the classification of pulse parameters.
2.3 rabbits, dogs intravenous infusion of noradrenaline a taut pulse model in rabbits, dogs underwent neck surgery exposed carotid artery, to breathe smoothly by two experienced doctors with double blind pulse taking, confirmed after using pulse pulse recorded by carotid pulse, then intravenous infusion to norepinephrine, again by two experienced doctors take double blind veins, identified as string pulse recorded after rabbit carotid pulse at the same pressure, dog's neck, elbow and femoral arterial Electropulsography and time domain analysis, do the classification of pulse parameters.
3 Results
3.1 NX - more than 3 instrument black probe 1 weight and electrical pressure value R = 0.9993; NX 3 type multifunctional sphygmograph black probe 2 weight and electrical pressure value R value is 0.9997, NX - 3 type multifunctional sphygmograph purple probe weight between electrical and pressure R value of 0.9999. three has a good linear relationship.
3.2 rabbits, dogs with the pulse position of teaching
The 8.92 + 0.12cm femoral artery pulsation in the knee joint of 3.2.1 rabbit is the close pulse
3.2.2 canine femoral artery and interfemoral crossing
3.3 rabbit, the sensation of the canine chord femoral artery
3.3.1: the pulse of a rabbit: in that, in the strong, weak anti refetch; a 17 ~ 18; pulse and strong sense of the string pulse refers to: the rate of 14 to 15, pulse position is shallow, long pulse shape, pulse potential at higher stress degree, give a person "such as the string" feeling. The pain type string pulse time lasting 30.07 + 4.06 minutes norepinephrine type string pulse has been in continuous infusion process.
3.3.2 dogs: a 8 pulse to 9; in both, in the strong, weak anti refetch; pulse flat and powerful; the pulse string pulse when the feeling of finger and rabbit string pulse similar to that of a 7 to 8 to, pulse light, pulse shape, pulse ratio in rabbits even the more powerful pulse, pulse tension high, "straight to long, such as

【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

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