小鼠Allo-HSCT后T淋巴细胞及其亚群组织分布的动态变化与GVHD关系

发布时间：2018-01-02 11:35

本文关键词：小鼠Allo-HSCT后T淋巴细胞及其亚群组织分布的动态变化与GVHD关系　出处：《第二军医大学》2005年博士论文　论文类型：学位论文

【摘要】：目的:深入研究T淋巴细胞亚群参与GVHD发生的机制,为防治GVHD的研究提供技术平台和理论基础。方法:用免疫磁珠阴性分选方法从转eGFP基因的C57/BL小鼠脾细胞中分离CD4~+T淋巴细胞、CD8~+T淋巴细胞,并输入经致死量全身照射的BALB/C小鼠体内制作GVHD模型。荧光显微镜观察受体鼠组织中eGFP~+细胞的分布、扩增情况。流式细胞仪检测T淋巴细胞表面分子,ELISA法检测细胞因子的动态变化。结果:CD4~+T淋巴细胞或(和)CD8~+T淋巴细胞均可诱发GVHD。移植后供体T淋巴细胞在肝、皮肤、肠、肺等组织中均呈逐渐增多趋势,而脾中呈逐渐减少趋势。T淋巴细胞表面CD25及受体鼠外周血IL-2水平在移植早期短期高峰表达,后迅速下降。结论:CD4~+T或CD8~+T淋巴细胞单独均可诱发GVHD。T淋巴细胞进入受体后先进入淋巴器官,激活扩增再移出淋巴器官,进入多种受体组织。IL-2及其受体CD25分子主要在移植早期参与GVHD。
[Abstract]:Objective: to investigate the role of T lymphocyte subsets in the pathogenesis of GVHD. Methods: CD4T lymphocytes were isolated from spleen cells of C57 / BL mice transformed with eGFP gene by immunomagnetic bead negative sorting method. CD8 ~ T lymphocytes were infused into BALB/C mice to make GVHD model. The distribution of eGFP~ cells in recipient mice was observed by fluorescence microscope. Amplification. Surface molecules of T lymphocytes were detected by flow cytometry. ELISA assay was used to detect the dynamic changes of cytokines. Results the donor T lymphocytes were in the liver and skin after transplantation. The expression of CD25 on the surface of T lymphocytes and IL-2 in peripheral blood of recipient mice was peak in the early stage of transplantation. Conclusion GVHD.T lymphocytes can be induced to enter the receptor and enter the lymphoid organs first by activation amplification and then out of the lymphoid organs by either CD4T or CD8T alone. IL-2 and its receptor CD25 molecules were mainly involved in the early stage of transplantation.
【学位授予单位】：第二军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392

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