内脏初级传入神经和化学表型的多重性

发布时间：2018-01-02 12:09

本文关键词：内脏初级传入神经和化学表型的多重性　出处：《新疆医科大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的:探讨肠神经系统的内脏初级传入神经元形态和神经元的化学表型,以此确定内脏伤害性刺激感受器的神经化超敏作用,为寻找伤害性刺激感受器的化学标记物和探讨内脏感觉超敏的神经机制。方法:利用免疫荧光细胞化学技术对BALBc小鼠的肠神经系统小肠和结肠远端的粘膜下神经丛(SMP)和肌间神经丛(MP)的内脏初级传入神经元进行人类神经蛋白(HU蛋白),细胞内钙阻断蛋白(Calretinin)和植物凝集素B4(IB4)的化学标记。结果:人类神经蛋白能标记所有的内脏初级传入神经元,Hu蛋白被认为是神经元的常规阳性标记物。显示细胞体形态和胞体的全浆性表达。Calretinin免疫阳性反应作为内脏初级传入神经元的显著标记物。Calretinin能很好的显示细胞的形态,Calretinin标记的显著特点在于整个细胞以及树突均匀地分布阳性反应地标记物。Calretinin标记的阳性神经元显示细胞体和树突,Hu蛋白标记的神经元表面光滑,而Calretinin免疫阳性神经元表面有突起,免疫反应阳性标记部位从根本把两者区分开。而植物凝集素B4免疫反应阳性神经元仅标记细胞膜和胞内颗粒状物质。IB4免疫阳性部位发生在细胞膜的表面和细胞内一侧的高尔基复合体,形成薄层的细胞膜表面阳性和胞浆内一侧的颗粒状阳性物质。IB4的细胞膜表达从根本上区别于Calretinin免疫反应阳性的全浆性和胞体表面的多突起表达。三种不同的阳性标记物,免疫反应部位既有雷同又有区别,使得免疫反应阳性神经元的定性有了确切的保证。通过三种不同的阳性标记物来发现和确认内脏伤害性刺激感受器的化学表型。结论:植物凝集素B4能与细胞膜上的碳糖类化合物特异性的结合,作为选择性细胞靶标来证实伤害性刺激信息由内脏初级传入神经元的传导。在小鼠的内脏神经系统,IB4免疫反应的阳性神经元,能够被确定为内脏伤害性刺激感受器的初级传入。IB4标记的阳性神经元对内脏伤害性刺激的感受触发了伤害性刺激过程的启动。内脏的高敏性是肠易激综合症(IBS)的主要发病机制,肠道感觉的高敏性和初级传入神经冲动地异常在IBS发病中起重要作用,胃肠动力的变化被认为是IBS最基本的病理生理学异常。在急性疼痛过程中,阐明了痛觉神经元的高敏化机制。
[Abstract]:Objective: To investigate the chemical phenotype of the enteric nervous system visceral afferent neurons and neuron morphology, in order to determine the visceral nociceptive stimulation receptor nerve ultra sensitive function, neural system for chemical markers for nociception and discussion of visceral stimulation hypersensitivity. Methods: BALBc mice intestinal nervous system the small intestine and the distal colon by fluorescent immunocytochemistry in the submucosal plexus (SMP) and myenteric plexus (MP) of primary afferent neurons of human visceral nerve protein (HU protein), intracellular calcium blocker protein (Calretinin) and lectin B4 (IB4) chemical markers. Results: human neuroprotein can mark all the visceral afferent neurons, Hu protein is considered normal positive markers of neurons. Show the morphology and cell body expression of.Calretinin immunoreactivity in the cytoplasm Visceral primary afferent neurons were markers of.Calretinin can show the cell shape is very good, the remarkable characteristics of Calretinin labeled positive neurons and dendritic cells in the uniform distribution of positive markers of.Calretinin markers showed cell bodies and dendrites of neurons, surface Hu protein and Calretinin immunoreactive neurons in smooth surface processes. Immunoreactive markers to distinguish the two parts from the root. The Golgi complex side lectin B4 immunoreactive positive neurons only plant cell membrane and intracellular markers of.IB4 immune positive granular material occurs in the cell membrane surface and inside the cell, the cell membrane formed on the side surface of the cell membrane and cytoplasm positive in the granular layer the expression of.IB4 positive material fundamentally different from the Calretinin immunoreactive pulp and cell surface The expression of surface protrusions. Three different positive markers, immune response at the site of both identical and difference, the qualitative immunoreactive neurons have a guarantee. By three different positive markers to find and confirm the visceral nociceptive stimulation receptor phenotype. Conclusion: chemical plant lectin B4 carbon specific carbohydrate compounds combined with the cell membrane, as a selective target cell to confirm the nociceptive information from the visceral afferent neuron conduction. In the visceral nervous system in mice, IB4 immunoreactive positive neurons, can be identified as visceral noxious stimulation receptor positive neurons in primary afferent.IB4 mark of visceral nociceptive triggered nociceptive process started. Visceral hypersensitivity is irritable bowel syndrome (IBS) is the main pathogenesis of gut feeling The abnormality of high sensitivity and primary afferent nerve impulse plays an important role in the pathogenesis of IBS. The change of gastrointestinal motility is regarded as the most basic pathophysiological abnormality of IBS. In the process of acute pain, the hyperalgesia mechanism of pain neurons is elucidated.

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R322

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