bFGF调节人内皮细胞ICAM-1表达的研究

发布时间：2018-01-02 18:13

  本文关键词：bFGF调节人内皮细胞ICAM-1表达的研究　出处：《中国医科大学》2006年硕士论文　论文类型：学位论文

  更多相关文章： 创伤修复 碱性成纤维细胞生长因子 内皮细胞 ICAM-1

【摘要】：bFGF调节人内皮细胞ICAM-1表达的研究 前言 创伤是广泛危害人类的“发达社会疾病”,深入研究创伤后的组织修复问题依然是医学领域的重点。创伤修复是一个复杂而有序的生物学过程,涉及多种炎症细胞、修复细胞、炎症介质、生长因子和细胞外基质等成分的共同参与。在组织修复研究中,各种生长因子在其中的作用及调控机制成'为现代创伤修复研究的热点。成纤维细胞生长因子(FGF),尤其是碱性成纤维细胞生长因子(bFGF)具有广泛的生物学活性,是伤口愈合过程中所有相关细胞的促有丝分裂原、化学趋化及调节蛋白,可以影响创伤修复的整个过程。目前有关于bFGF促进内皮细胞(EC)增殖、调节EC蛋白表达、分泌,促进血管新生、肉芽组织形成方面的研究报道,但有关bFGF调节内皮细胞黏附分子表达,影响早期炎症反应的研究则报道很少,对EC细胞间黏附分子-1(ICAM-1)表达的影响,国内尚未见报道。 本实验通过研究bFGF调节人脐静脉内皮细胞(HUVEC)ICAM-1表达的时间效应,及糖皮质激素地塞米松(Dex)对bFGF调节ICAM-1表达的影响,来阐述bFGF影响修复早期炎症反应,进而促进创伤修复的可能机制。 方法 1.EC的培养:采用本室改进的Jaffe氏法进行HUVEC培养。经免疫荧光检测Ⅷ因子抗原阳性,鉴定为血管内皮细胞,采用1-3代EC进行实验,待细胞达亚融合状态后,换无血清培养液24h,之后施加各实验因素。2.HUVEC ICAM-1表达的测定:实验分组:(1)不同时间组:对照组(无血清DMEM);实验组:bFGF(500ng/ml)12h、16h、24h、48h组(2)不同因素组(16h):对照组(无血清DMEM);bFGF(500ng/ml)、Dex(0.1μmol/L)、bFGF+Dex组。采用免疫细胞化学法(SP法)及流式细胞仪检测法分析EC
[Abstract]:Regulation of ICAM-1 expression in Human Endothelial cells by bFGF Foreword Trauma is a "developed social disease" that harms human beings extensively. It is still the focus of medical field to study the tissue repair problem after trauma. Wound repair is a complicated and orderly biological process. Involved in a variety of inflammatory cells repair cells inflammatory mediators growth factors and extracellular matrix and other components involved in tissue repair research. The role and regulatory mechanism of various growth factors are the focus of modern wound repair research. Fibroblast growth factor (FGFs). In particular, basic fibroblast growth factor (bFGF) has a wide range of biological activities, and is a mitogen, chemotaxis and regulatory protein of all the related cells during wound healing. BFGF promotes endothelial cell proliferation, regulates the expression and secretion of EC protein, promotes angiogenesis and granulation tissue formation. However, there are few reports about the effect of bFGF on the expression of adhesion molecules in endothelial cells, and on the expression of intercellular adhesion molecule-1 (ICAM-1) in EC. No reports have been reported at home. The aim of this study was to investigate the effect of bFGF on the expression of ICAM-1 in human umbilical vein endothelial cells (HUVECs). The effect of glucocorticoid dexamethasone Dexon on the expression of ICAM-1 by bFGF was used to elucidate the possible mechanism of bFGF affecting the early inflammatory response and promoting wound repair. Method 1. EC culture: HUVEC culture was carried out by modified Jaffe's method in our laboratory. Factor 鈪，

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