P物质与神经生长因子对正常人外周血中NK细胞杀伤活性的影响

发布时间：2018-01-02 21:40

本文关键词：P物质与神经生长因子对正常人外周血中NK细胞杀伤活性的影响　出处：《中国医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：前言人们在观察应激对机体免疫功能影响时发现,应激会导致免疫功能低下、自然杀伤细胞活性受到抑制、淋巴细胞总数减少、单核细胞数减少等等。神经内分泌系统对机体免疫功能具有重要的调控作用,而经典神经递质、神经肽则是直接作用于免疫活性细胞和其他参与免疫反应细胞的重要调节物质。以往有学者报道,SP可促进增殖细胞B产生抗体,并刺激单核细胞产生某些细胞因子共同参与免疫调节作用。有文献指出,无论是体外孵育还是体内给药,SP均可增强小肠上皮淋巴细胞中NK细胞的活性。体内给药能增加脾脏细胞的杀伤力。并且Hart R等发现SP能增加人外周血单核样细胞的IgM及干扰素的分泌。以上资料均表明P物质是体内重要的调节性多肽,并且对免疫功能具有调节作用。神经生长因子(nerve growh factor NGF)是一种嗜神经多肽,亦是发现最早和已知最典型的神经营养因子。其作为一种为感觉神经和交感神经元存活分化和功能所必需的神经营养多肽在神经系统的发育过程中起关键性作用。随着研究的发展和深入,科学家们发现神经生长因子对免疫系统及免疫应答的调节也产生重要影响。 NK细胞是机体十分重要的细胞群体,它几乎参与了免疫系统的发生、发展及效应等重要环节的调节过程。目前,对NK细胞的研究远少于T细胞和B细胞,而有关神经-内分泌-免疫网络中NK细胞所担任的角色、功能变化的完整的文献报道甚少。Hellstrand等发现如果NK细胞经过低浓度的肾上腺素预处理,可以提高NK细胞的活性,但如果10~(-6)M浓度的肾上腺素与NK细胞同育,则NK细胞活性会大大下降。因而,NK细胞对应激原的反应是最敏感的。神经生长因子(NGF)和P物质(SP)同属多肽类物质。众多资料表明,二者对免疫功能有明显的调节作用。但关于二者在体外对人外周血单个核细胞活性的影响均未见相关报道。本实验研究了SP和NGF对人外周血中
[Abstract]:Foreword When people observe the effect of stress on immune function, it is found that stress can lead to low immune function, inhibition of natural killer cell activity and decrease of total number of lymphocytes. The neuroendocrine system plays an important role in regulating the immune function of the body, while the classic neurotransmitter. Neuropeptides are important regulatory substances acting directly on immunoreactive cells and other immunoreactive cells. In the past, it was reported that SP can promote the production of antibodies in proliferative cells. And stimulate monocytes to produce some cytokines involved in the immune regulation. It has been pointed out that whether in vitro incubation or in vivo administration of drugs. Sp could enhance the activity of NK cells in intestinal epithelial lymphocytes. In vivo administration of SP could increase the cytotoxicity of spleen cells and Hart. It was found that SP could increase the secretion of IgM and interferon in human peripheral blood mononuclear cells. All of the above data indicated that substance P was an important regulatory polypeptide in vivo. Nerve growth factor (NGF) nerve growh factor (NGF) is a neurotropic polypeptide. It is also the earliest and most typical known neurotrophic factor. As a neurotrophic peptide necessary for the survival, differentiation and function of sensory and sympathetic neurons, neurotrophic peptides play a key role in the development of the nervous system. With the development and development of the research. Scientists have found that NGF also plays an important role in regulating the immune system and immune response. NK cells are a very important cell population in the body, which almost participate in the development, development and regulation of the immune system. At present, the study of NK cells is much less than that of T cells and B cells. The role of NK cells in neuro-endocrine-immune networks. There are few reports of functional changes in the literature. Hellstrand et al found that NK cell activity could be increased if NK cells were pretreated with low concentration of epinephrine. However, if the concentration of epinephrine and NK cells were incubated with 10 ~ 10 ~ (-6) 渭 M, the activity of NK cells would decrease greatly, so the response of NK cells to stressors was the most sensitive. Nerve growth factor (NGF) and substance P (substance P) are polypeptides. The effects of SP and NGF on the activity of human peripheral blood mononuclear cells (PBMC) in vitro were not reported. The present study was conducted to study the effects of SP and NGF on the activity of human peripheral blood mononuclear cells (PBMC).
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392.12

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