胎儿胸腺发育中弥散神经内分泌细胞的表达及其意义

发布时间：2018-01-03 00:23

本文关键词：胎儿胸腺发育中弥散神经内分泌细胞的表达及其意义　出处：《暨南大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：检测胎儿胸腺发育中弥散神经内分泌细胞(diffuse neuroendocrinecells，DNES细胞)分布和表达特征，探讨DNES细胞对胎儿胸腺的生物学作用及其意义，为神经内分泌免疫网络理论提供形态学资料。方法： 15～40周胎儿胸腺共27例，分别进行HE染色、免疫组织化学染色和双重免疫荧光染色。抗嗜铬素A(Chromogranin A，CgA)、抗神经元特异性烯醇化酶(Neuron specidic-enolase，NSE)记DNES细胞，抗生长抑素(Somatostatin，SS)和抗血管活性肠肽(Vasoactive intestnal peptide，VIP)检测DNES细胞的内含物。通过图像分析系统检测各胎龄组DNES细胞的面数密度。结果： 1．免疫组化染色 CgA和NSE阳性细胞在15～38周胎儿胸腺中均表达，27～28周前随胎龄增加表达增多，后减少，39周及40周未见表达。两者表达特征相似，它们主要散在分布于胸腺髓质胸腺细胞之间，细胞呈圆或椭圆形；分布于胸腺小体周边，细胞呈圆、椭圆或三角形。SS和VIP阳性细胞在胸腺发育过程中15周即表达持续至40周，在17周、18周表达较旺盛，分布于胸腺髓质及皮髓交界处，呈圆或椭圆形，大小如胸腺细胞或略大。胸腺皮髓质交界处和髓质胸腺上皮细胞VIP和SS免疫反应阳性。 2．免疫荧光双标胎儿胸腺发育中CgA、NSE、SS和VIP均有表达，分布及形态特征同免疫组化所示相似。CgA和NSE，CgA和SS，CgA和VIP阳性细胞部分重叠。结论： 1．胎儿胸腺发育中DNES细胞发育活跃，尤其在胚胎发育中期，表明其可能对胸腺发育和功能建立具有重要影响。
[Abstract]:Objective: The distribution and expression of diffuse neuroendocrine cells (diffuse neuroendocrine cells) during fetal thymus development were detected. To investigate the biological effect of DNES cells on fetal thymus and its significance, and to provide morphological data for neuroendocrine immune network theory. Methods: A total of 27 cases of fetal thymus at 1540 weeks were stained with HE, immunohistochemistry and double immunofluorescence staining. DNES cells were recorded by CGA, anti-neuron-specific enolase (Neuron specidic-enolase NSE). Antisomatostatin (SS) and vasoactive intestnal peptide (Vasoactive intestnal peptide). The surface number density of DNES cells in each gestational age group was detected by image analysis system. Results: 1. Immunohistochemical staining The expression of CgA and NSE positive cells in the thymus of the fetus at 15 ~ 38 weeks was increased with the increase of gestational age before 2728 weeks, and then decreased. No expression was found at 39 weeks and 40 weeks. The expression characteristics were similar. They were mainly scattered between thymus medulla thymocytes and the cells were round or oval. The cells were round around the thymus corpuscles. The positive cells of ellipse or triangle. SS and VIP expression lasted from 15 weeks to 40 weeks during the development of thymus, and the expression was strong at 17 weeks and 18 weeks. It is located at the junction of thymus medulla and medulla, and is round or ellipse, with the size of thymocyte or a little larger. The VIP and SS immunoreactivity of thymic medullary junction and medullary thymic epithelial cells are positive. 2. Double immunofluorescence labeling The expression, distribution and morphological characteristics of VIP and CgA in fetal thymus were similar to those of CGA, CGA and SS in the development of fetal thymus. CgA and VIP positive cells overlapped partly. Conclusion: 1. DNES cells are active in the development of fetal thymus, especially in the middle stage of embryonic development, indicating that it may play an important role in thymus development and function establishment.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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