大鼠脑组织嘌呤核苷酸分解代谢关键酶及μ受体基因表达研究

发布时间：2018-01-03 02:25

  本文关键词：大鼠脑组织嘌呤核苷酸分解代谢关键酶及μ受体基因表达研究　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

  更多相关文章： 腺苷酸脱氨酶 黄嘌呤氧化酶 μ-R 脑组织

【摘要】：为了研究大鼠脑组织不同区域核酸分解代谢关键酶腺苷脱氨酶(ADA)和黄嘌呤氧化酶(XO)活性和基因表达是否存在差异,进而研究给予处理后脑组织不同区域是否存在腺苷脱氨酶(ADA)和黄嘌呤氧化酶(XO)基因表达差异提供理论依据,我们取雄性大鼠脑组织,测定其酶活性,并采用RT-PCR及PCR技术测定其的ADA和XO mRNA的相对含量。本研究结果显示:1.大鼠脑组织不同区域ADA酶活性存在差异,顶叶、颞叶、脑干、海马ADA酶活性较高;2.大鼠脑组织不同区域XO酶活性存在差异,顶叶、颞叶、脑干、海马ADA酶活性较高;3.大鼠脑组织不同区域ADA mRNA的相对含量存在差异,mRNA含量与酶活性变化基本一致;4.大鼠脑组织不同区域XO mRNA的相对含量存在差异, mRNA含量与酶活性变化基本一致;5.大鼠脑组织不同区域μ-R mRNA的相对含量存在差异,中脑、脑干、丘脑、海马mRNA相对含量较高。以上结果表明,大鼠组织嘌呤核苷酸分解代谢酶及μ-R的基因表达存在差异。
[Abstract]:In order to study the difference in activity and gene expression of adenosine deaminase (ADAA) and xanthine oxidase (XO), the key enzymes of nucleic acid catabolism in different regions of rat brain were studied. Then to study whether there are differences in the expression of adenosine deaminase (ADAA) and xanthine oxidase (XO) gene in different regions of brain tissue after treatment, we take the brain tissue of male rats. The enzyme activity was determined. The relative contents of ADA and XO mRNA were determined by RT-PCR and PCR techniques. The results showed that there were differences in ADA enzyme activity in different regions of brain tissue of the rat and parietal lobe. The activity of ADA was higher in temporal lobe, brain stem and hippocampus. 2. The activity of XO enzyme was different in different regions of rat brain. The activity of ADA was higher in parietal lobe, temporal lobe, brain stem and hippocampus. 3. The relative content of ADA mRNA in different regions of brain tissue of rats was similar to that of enzyme activity. 4. The relative content of XO mRNA in different regions of brain tissue of rats was different, and the content of mRNA was basically consistent with the change of enzyme activity. 5. The relative content of 渭 -R mRNA in different regions of rat brain was different. The relative content of mRNA was higher in midbrain, brain stem, thalamus and hippocampus. The gene expression of purine nucleotide catabolism enzyme and 渭-R in rat tissues was different.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R341

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