大鼠在获得空间辨别性学习记忆时海马结构形态学可塑性研究

发布时间：2018-01-03 03:04

本文关键词：大鼠在获得空间辨别性学习记忆时海马结构形态学可塑性研究　出处：《暨南大学》2005年硕士论文　论文类型：学位论文

【摘要】：探讨大鼠在获得空间辨别性学习记忆功能时其海马结构的形态学可塑性及可塑性与有关神经因子表达之间的关系。方法水迷宫训练建立空间辨别性学习记忆动物模型后,用Timm染色方法、免疫组织化学方法和Western-blot方法检测模型大鼠、游水大鼠和对照大鼠海马结构神经形念学可塑性反应—神经纤维出芽、以及与神经可塑性有关的导向分子ephrin-A3表达差异。结果 1.建立空间辨别性学习记忆动物模型的结果:大鼠经过水迷宫训练到第7d时,其平均潜伏期、平均运行时间以及正确率三项指标即可到达规定指标; 2.各组大鼠海马结构可塑性的结果:(1)模型组大鼠海马结构内Timm染色显示出芽的神经纤维主要分布在CA3区多形层,并且出芽反应以14d最旺盛;9只模型大鼠中有8只显示神经纤维出芽(88.8%);(2)9只游水组大鼠中有2只在海马CA3区多形层分布着出芽的神经纤维(22.2%),7只大鼠未见出芽反应;(3)对照组9只大鼠均未见海马CA3区多形层有出芽的神经纤维分布。 3.各组大鼠海马结构内ephrin-A3的定位和定量结果:在定位方面,模型组、游水组和对照组大鼠海马结构各亚区和各层均可见ephrin-A3免疫反应阳性产物分布,但模型组大鼠海马CA3区辐射层反应尤为明显。在定量方面,模型组大鼠海马结构内ephrin-A3含量明显高于游水组和对照组的(P0.01),游水组的比对照组的亦高,差异有显著性(P0.01);模型组内14d和21d比7d高,差异有显著性(P0.01)。结论 1.用水迷宫训练大鼠,可以建立空间辨别性学习记忆模型; 2.获空间辨别性学习记忆功能的大鼠海马结构发生了神经纤维出芽可塑性: 3.ephrin-A3免疫反应阳性物与海马结构神经纤维出芽有相关关系。
[Abstract]:To investigate the relationship between morphological plasticity and plasticity of hippocampal structure and the expression of related neurofactors during the acquisition of spatial discriminant learning and memory function in rats. Method After establishing the animal model of spatial discriminant learning and memory by water maze training, the model rats were detected by Timm staining, immunohistochemistry and Western-blot. The neuronal plasticity of hippocampal formation in swimming rats and control rats was different from that in control rats. The neuronal fibers sprout and the ephrin-A3 expression of guiding molecules related to neural plasticity were different between the swimming rats and the control rats. Results 1. The results of establishing the animal model of spatial discriminant learning and memory: the average latency of the rats after water maze training was 7 days. The average running time and the correct rate can reach the specified index. 2. The results of the plasticity of hippocampal formation in each group: 1) the Timm staining in the hippocampal structure of rats in the model group showed that the budding nerve fibers were mainly distributed in the polymorphic layer of the CA3 area. The sprout reaction was the strongest in 14 days. Of the 9 model rats, 8 showed nerve fiber sprouting and 88. 8%. Two of 9 rats in the swimming group had no budding reaction in the polymorphic layer of hippocampal CA3. 3) in the control group, no budding nerve fibers were found in the polymorphic layer of the hippocampal CA3 region. 3. Localization and quantitative results of ephrin-A3 in hippocampal formation of rats in each group: in terms of localization, model group. The distribution of ephrin-A3 immunoreactive products was observed in all subregions and layers of hippocampal formation of rats in swimming group and control group, but the radiation layer reaction in CA3 area of hippocampus in model group was more obvious. The content of ephrin-A3 in the hippocampal structure of the model group was significantly higher than that of the swimming group and the control group (P 0.01), and that of the swimming group was also higher than that of the control group (P 0.01). In the model group, 14 d and 21 d were higher than 7 d, the difference was significant (P 0.01). Conclusion 1. The model of spatial discriminative learning and memory can be established by training rats with water maze. 2. Neural fiber budding plasticity occurred in the hippocampal structure of rats with spatial discriminant learning and memory function. 3. The immunoreactive substance of ephrin-A 3 was correlated with the sprouting of hippocampal nerve fibers.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R322

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