去甲肾上腺素对外周细胞炎性因子表达的影响

发布时间：2018-01-04 01:34

本文关键词：去甲肾上腺素对外周细胞炎性因子表达的影响　出处：《南华大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的:观察去甲肾上腺素作用THP-1细胞、THP-1巨噬细胞和内皮细胞后CD137、IL-6以及HMGB1基因表达的影响。方法:对THP-1细胞、THP-1巨噬细胞和内皮细胞给予应激浓度的去甲肾上腺素刺激(剂量10μmol/L,时间1~24h),运用逆转录-多聚酶链式反应检测CD137、IL-6和HMGB1 mRNA的表达,酶联免疫吸附试验检测IL-6蛋白的表达,Western印迹检测CD137蛋白的表达。结果: 10μmol/L去甲肾上腺素作用THP-1细胞、THP-1巨噬细胞和内皮细胞1~24h后,其CD137、IL-6的mRNA和蛋白水平均上升,HMGB1 mRNA表达上升(p0.05)。结论:应激浓度的去甲肾上腺素能不同程度的上调THP-1细胞、THP-1巨噬细胞和内皮细胞CD137、IL-6和HMGB1基因的表达。
[Abstract]:Objective: To observe the effect of norepinephrine on the expression of CD137, IL-6 and HMGB1 gene in THP-1 cells, THP-1 macrophages and endothelial cells.
Methods: THP-1 THP-1 cells, macrophages and endothelial cells to stress concentration of norepinephrine (dose 10 mol/L, time 1~24h, CD137) were detected by reverse transcriptase polymerase chain reaction, the expression of IL-6 and HMGB1 mRNA, the expression of IL-6 protein by enzyme-linked immunosorbent assay, the expression of CD137 protein was detected by Western blotting.
Results: the effect of 10 mol/L of norepinephrine on THP-1 cells, THP-1 macrophages and endothelial cells after 1~24h increased mRNA and protein levels of CD137, IL-6, and HMGB1 mRNA expression increased (P0.05).
Conclusion: the stress concentration of norepinephrine can increase the expression of CD137, IL-6 and HMGB1 genes in THP-1 cells, THP-1 macrophages and endothelial cells in varying degrees.

【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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