中华按蚊防御素基因的克

发布时间：2018-01-05 20:20

本文关键词：中华按蚊防御素基因的克隆、表达及重组蛋白的纯化与生物活性的初步评价　出处：《第一军医大学》2005年硕士论文　论文类型：学位论文

【摘要】：研究背景:以蚊为媒介的传染病,如:疟疾(malaria)、流行性乙型脑炎(epidemic encephalitis B)、丝虫病(filariasis)、登革热(dengue fever)等,是当今社会严重威胁人民生命健康的重要疾病。WHO公布的“十大重点防治的疾病”中,疟疾和丝虫病均位于其中,疟疾更是世界上感染率最高、死亡人数最多的疾病之一。根据2003年国家卫生部统计资料,我国疟疾的发病率为3.00/10万,世界上每年的疟疾死亡人数达270万。登革热的发病率近年也有逐年递增的趋势,并常有局部暴发流行的报道。因此,蚊及蚊媒病的防治仍是迫切需要解决的问题,控制蚊虫是减少蚊媒病的一种有效方法。昆虫是世界上最大的生物种群,为了适应不同的生态环境,建立了自己高效独特的免疫系统,抗菌肽(antibacterial peptides,ABP)就是其免疫系统中的关键成分。对于外来病原的感染,蚊虫会通过激活其内源的细胞和体液免疫来形成有效的防御体系。目前最为人们所关注的是富含半胱氨酸的一类多肽,称为“防御素”(defensin)。蚊虫的防御素基因首先从埃及伊蚊中克隆,体外实验表明防御素可以抗革兰氏阴性细菌和革兰氏阳性细菌,对真菌、病毒、原虫和肿瘤细胞均有杀伤作用。当前不少病原菌对已有抗生素逐渐产生抗药性,而新的抗生素发现又极为困难,防御素有可能成为新型的抗菌素、抗病毒素以及抗肿瘤药物。中华按蚊是我国疟疾传播的重要媒介,目前有关中华按蚊的防御素研究国内外尚未见报道。本研究尝试克隆中华按蚊defensin基因的编码序列,并对其在
[Abstract]:Background: mosquito-borne infectious diseases, such as malarial encephalitis B, epidemic encephalitis B). Filariasisof filariasis, dengue fever and so on. Nowadays, malaria and filariasis are among the ten most important diseases that threaten people's life and health. The infection rate of malaria is the highest in the world. According to the statistics of the Ministry of Health in 2003, the incidence of malaria in China is between 300 and 100 thousand. The annual death toll from malaria in the world is 2.7 million. The incidence of dengue fever has been increasing in recent years, and local outbreaks are often reported. The control of mosquito and mosquito vector disease is still an urgent problem to be solved, and mosquito control is an effective method to reduce mosquito vector disease. Insects are the largest biological population in the world. In order to adapt to different ecological environments, insects have established their own highly efficient and unique immune system. Antimicrobial peptide (ABP) is a key component of its immune system. Mosquitoes form an effective defense system by activating their endogenous cellular and humoral immunity. The defensin gene of mosquitoes was first cloned from Aedes aegypti. Experiments in vitro showed that defensin could resist gram-negative bacteria and gram-positive bacteria against fungi. Viruses protozoa and tumor cells all have killing effects. At present many pathogens gradually develop resistance to existing antibiotics and the discovery of new antibiotics is extremely difficult. Defensins may become a new type of antibiotics. Antiviral and anti-tumor drugs. Anopheles sinensis is an important vector for malaria transmission in China. At present, the defensins of Anopheles sinensis have not been reported at home and abroad. This study attempts to clone the encoding sequence of defensin gene of Anopheles sinensis.
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R384

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