经颅微电流刺激定位大鼠皮层面部运动代表区

发布时间：2018-01-06 16:29

本文关键词：经颅微电流刺激定位大鼠皮层面部运动代表区　出处：《山东大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：本研究采用经颅微电流刺激的方法，精确定位大鼠皮层面部运动代表区，为大鼠外周面神经与其中枢面部运动皮层之间相互联系的深入研究提供了基础资料。研究方法：选用25只250～350g雌性成年Wistar大鼠，分别在浅麻醉(盐酸氯胺酮)下，利用脑立体定位仪，垂直大脑皮层表面插入微电极至皮层下1.5～1.7mm，每次给以300Hz、0.25ms的负相方波脉冲30ms，相邻刺激点相距500μm，每两次刺激间隔至少5s。观察大鼠的面部运动(眨眼、胡须运动、耳廓运动及口唇运动)，并通过引导相应运动部位肌电图的方法确定各皮层刺激点的刺激阈电流强度。以前囟点为原点，头尾的中线为y轴，通过原点的中线垂直线为x轴确立坐标系，记录各相关刺激点的坐标值，根据各刺激点诱发的运动描绘出大鼠面部皮层运动代表区图谱。结果：大鼠皮层面部运动代表区由数个支配特定运动的形状不规则的皮层区域组成。各亚区之间相对独立，并不连续，躯体上相邻的部位其皮层运动代表区并不相邻。各亚区的分布在每个大鼠之间存在个体差异，但基本是一致的。其皮层代表区不仅分布于大鼠的运动皮层，还有很大部分与感觉皮层相重合。 1．胡须运动代表区有两部分：第一部分位于前内侧皮层，面积平均为9.5mm~2，该区域平均有效刺激点为38个。该区域的内侧部分在TI下诱发同侧胡须的运动，，平均面积为2.5mm~2，有效刺激点平均为10个，平均TI为28
[Abstract]:Objective: in this study, transcranial micro current stimulation was used to locate the cortex and facial motor area in rats. It provides basic data for further research on the interconnections between the peripheral facial nerve and the medial facial cortex.
Research methods: selection of 25 ~ 250 350g female adult Wistar rats were in light anesthesia (ketamine), using stereotaxic apparatus, the vertical surface of the cerebral cortex to subcortical microelectrode insertion of 1.5 ~ 1.7mm, each with 300Hz, 0.25ms negative square wave pulse 30ms, adjacent stimulation point 500 m apart two, each stimulus interval of at least 5S. to observe rat facial movements (blink, beard movement, ear movement and moving lips), and the corresponding parts of the motion guide EMG to determine the cortical stimulation threshold current intensity point. Fontanelle point as the origin, and line to the Y axis, the vertical line through the midline the origin of the establishment of coordinate system for the X axis, record the coordinates of each stimulus point, depict the facial motor cortex area of rats according to the stimulation induced by exercise.
Results: the cortical regions of facial motor cortex area consists of a number of specific control movement of irregular shape. Each sub region is relatively independent, not continuous, body adjacent parts of the cortical motor area is adjacent. The distribution of each sub region there are individual differences between each rat, but the basic is consistent. The motor cortex cortex not only distributed in the rat, and there is still a majority of the sensory cortex coincide.
1. beard motor area has two parts: the first part is located in the medial cortex, the area is 9.5mm~2 on average, the regional average effective stimulus point 38. The inner part of the area under the TI movement evoked by ipsilateral whiskers, the average area of 2.5mm~2, effectively stimulate the point average is 10, the average TI is 28

【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R322

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