白血病抑制因子、胞饮突与子宫内膜容受性关系的实验研究

发布时间：2018-01-07 19:14

本文关键词：白血病抑制因子、胞饮突与子宫内膜容受性关系的实验研究　出处：《郑州大学》2006年博士论文　论文类型：学位论文

【摘要】：胚胎种植(implantation)是一个复杂的生理过程。在此期间胚胎与子宫双方都处在生长发育的动态阶段，子宫内膜与胚胎相互识别、彼此相容，有侵入能力的胚胎和具有接受性的子宫内膜相互作用，发生一系列生理生化及形态改变，即双方建立起“对话”机制，胚胎才能在子宫内膜正常植入。在人和哺乳动物的正常生殖周期中，子宫内膜仅有一个极短的时期允许胚胎种植，这段时期称“种植窗期”，此时子宫内膜表现出最大的容受性。子宫内膜对胚胎的容受性是胚胎种植成功的关键，如果子宫内膜不适合胚胎着床将导致妊娠失败。目前国内外有关子宫内膜容受性的研究主要集中在生殖调控因子和内膜超微结构两方面。生殖调控因子有多种，如：白血病抑制因子(leukaemia inhibitory factor，LIF)、整合素家族(integrins)、胞饮突(pinopode)、肝素结合样表皮生长因子(heparin-binding epidermal growth factor，HB-EGF)、白细胞介素Ⅰ的Ⅰ型受体(IL-IRt-I)、粘液素-Ⅰ(MUC-Ⅰ)、一氧化氮合酶(NOS)、雌孕激素受体等。胞饮突的形成是子宫内膜超微结构中最具代表性的形态学改变。 LIF是一种多生物学功能的分泌型糖蛋白，为IL-6家族成员，具有广泛的生物学活性，最早发现它具有促进小鼠MI型白血病细胞分化并抑制其增殖而命名。近年来的研究表明LIF参与了生殖活动的各个环节，如促进卵泡的发育、胚胎的生长、分化及着床等，尤其是与胚胎种植关系密切。Stewart等采用基因重组技术，发现LIF基因缺陷的雌鼠能够排卵，其卵子亦能够受精，但胚泡不能正常植入及发育，若转移到野生型假孕鼠中，则胚泡能够正常植入和发育，提出LIF是动物胚泡植入过程所必需的。随后，LIF与胚胎的种植关系引起人们极大的兴趣，，成为研究的热点。人们对子宫内膜表达LIF的情况进行了大量研
[Abstract]:Embryo implantation (implantation) is a complex physiological process. During the embryo and the uterus are both at the dynamic stage of growth and development, mutual recognition, endometrium and embryo compatible invade ability of embryos and has accepted the interaction of endometrium, a series of physiological and biochemical and morphological changes, namely, the two sides established the "dialogue" mechanism, in order to endometrial normal embryo implantation. The normal reproductive cycle in human and mammals, endometrial only for a very short period to allow embryo implantation, this period is called "implantation window", the endometrial receptivity showed the largest. The endometrial receptivity to embryo is the key to the success of embryo implantation, if the endometrium is not suitable for embryo implantation will lead to pregnancy failure.
At present study on endometrial receptivity at home and abroad mainly focus on two aspects of reproductive control factors and endometrial morphology. There are many factors such as the regulation of reproduction: leukemia inhibitory factor (leukaemia inhibitory, factor, LIF), integrin family (integrins), pinopode (pinopode), heparin binding epidermal growth factor (heparin-binding epidermal growth factor, HB-EGF), type I receptor of interleukin 1 (IL-IRt-I), mucin 1 (MUC- 1), nitric oxide synthase (NOS), estrogen and progesterone receptors. Pinopode formation is the morphological changes of endometrial ultrastructure in the most representative.
LIF is a kind of biological function of secreted glycoprotein, is a member of the IL-6 family, has a wide range of biological activity, the earliest found that it can promote the differentiation of mouse MI leukemia cells and inhibit its proliferation and naming. Recent studies have shown that LIF is involved in all aspects of reproductive activities, such as the promotion of follicular development, embryo the growth, differentiation and implantation, especially with embryo implantation is closely related to.Stewart using the gene recombination technique, found that LIF deficient female rats can ovulation, the egg can also be fertilized, but not normal blastocyst implantation and development, if transferred to the wild type of pseudopregnant mice, embryo implantation and development is normal, put forward LIF is required for animal implantation. Subsequently, LIF and embryo implantation relations aroused great interest, has become a hot research topic. People on the endometrial expression of LIF were a lot study

【学位授予单位】：郑州大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R321

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