微囊化稳定表达人血管内皮生长因子细胞株的构建

发布时间：2018-01-08 10:26

本文关键词：微囊化稳定表达人血管内皮生长因子细胞株的构建　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

【摘要】：组织工程血管化问题是组织工程的限速步骤,再生的组织器官如果不能快速、适当地在宿主体内在血管化,最终将导致其坏死,无法代替病损组织发挥生物学作用。人血管内皮生长因子是近年来血管化研究中的热点问题。1989年,Ferrara等从牛垂体滤泡星状细胞体外培养液中首次分离到血管内皮细胞生长因子(VEGF)蛋白。它是一类内皮细胞特异性的有丝分裂原,具有强烈诱导血管生成的作用,同时能够增加微静脉、小静脉的通透性,因此,又称为血管通透因子(VPF)。本实验通过分子克隆技术从人胎肝中调取hVEGF165的cDNA,构建重组真核表达载体,稳定转染小鼠成肌细胞c2c12。然而利用异种或异体细胞进行移植会产生强烈的免疫排斥反应。我们利用微囊化技术将细胞经微囊化包裹,从而制备微囊化稳定表达hVEGF165的细胞,以期应用于组织工程的器官再造或缺血组织。
[Abstract]:Vascular tissue engineering problem is the rate limiting step in tissue engineering and regeneration of tissues and organs if not fast, proper in vivo in blood vessels, will eventually lead to the necrosis, unable to replace the damaged tissue play a biological role. Vascular endothelial growth factor in angiogenesis of the hot issues in the.1989, Ferrara and so on bovine pituitary follicular stellate cells in vitro culture medium was first isolated vascular endothelial growth factor (VEGF) protein. It is a kind of endothelial cell specific mitogen induced angiogenesis, has a strong role, at the same time can increase the micro vein, small vein permeability, therefore, also known as vascular permeability factor (VPF).
This experiment by molecular cloning technique cDNA the transfer of hVEGF165 from human fetal liver, the recombinant eukaryotic expression vector and stably transfected mouse myoblast c2c12. but by heterogeneous or allogeneic cells transplantation can produce strong immune rejection. We use microencapsulation cells were encapsulated, thereby preparing microcapsule stability the expression of hVEGF165, which would be used in tissue engineering organ reconstruction or ischemic tissue.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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