2011年龙岩市流行株柯萨奇病毒A组16型VPl基因特征分析

发布时间：2018-01-08 20:19

本文关键词：2011年龙岩市流行株柯萨奇病毒A组16型VPl基因特征分析　出处：《中国病原生物学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的分析2011年龙岩市流行的柯萨奇病毒A组16(CVA16)分离株的分子生物学特征。方法对从龙岩市2011年散发的手足口病(HFMD)患者标本中分离CVA16病毒,采用逆转录-聚合酶链反应(RT-PCR)扩增VPl基因片段全长,并对扩增产物测序。根据VPl基因核苷酸序列与国内外其他CVA16毒株序列构建进化树,并进行核苷酸和氨基酸同源性分析。结果 4株龙岩分离株CVA16核苷酸同源性为99%~100%。比较推导的氨基酸序列,VP1不存在位点差异,氨基酸序列完全相同。对分离株病毒进行VP1核苷酸序列两两差异分析,与国内其他分离株VP1基因序列的一致性为87%~100%。确定4株CVA16病毒均属于B1b基因型。结论引起2011年龙岩市HFMD流行的CVA16病毒均为B1b基因型,与目前国内其他地区流行毒株高度同源。
[Abstract]:Objective to analyze the molecular biological characteristics of Coxsackie virus A (Coxsackie virus A) group 16 CVA16 isolated from Longyan City on 2011. Methods HFMDs from HFMD isolated from Longyan City on 2011 were analyzed. CVA16 virus was isolated from patient specimen. The full length of VPl gene fragment was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The amplified products were sequenced. The phylogenetic tree was constructed according to the nucleotide sequence of VPl gene and other CVA16 strains at home and abroad. The homology of nucleotide and amino acid was analyzed. Results the nucleotide homology of CVA16 in 4 Longyan isolates was 99100. The deduced amino acid sequence (VP1) had no site difference. The amino acid sequence was identical. The VP1 nucleotide sequence of the isolated virus was analyzed by pairwise differential analysis. The conformance of VP1 gene sequence with other isolates in China is 870.All of the four CVA16 viruses belong to the B1b genotype. Conclusion in 2011, the HFMD epidemic occurred in Longyan City. All VA16 viruses were B1b genotypes. It is highly homologous with other endemic strains in China.
【作者单位】：福建省龙岩市疾病预防控制中心;福建医科大学龙岩市疾病预防控制中心教学点;
【基金】：福建省卫生厅青年科研课题项目(2012-1-47)
【分类号】：R373.23
【正文快照】： ***近年来,以肠道病毒7l型(Enterovirus 71,EV71)和CVA16为主的肠道病毒(Enterovirus,EV)引起的手足口病(Hand,foot,and mouth disease,HFMD)在各地流行,2006年4月龙岩市新罗区某幼儿园曾发生一起由CVA16引起的手足口病暴发[1]。由于EV71感染除引起HFMD外还可引起较严重的并发

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