沙门菌MALDI-TOF-MS标准菌库的建立及应用研究

发布时间：2018-01-10 19:23

本文关键词：沙门菌MALDI-TOF-MS标准菌库的建立及应用研究　出处：《卫生研究》2014年01期 　论文类型：期刊论文

【摘要】：目的利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)构建标准沙门菌株数据库。方法利用MALDI-TOF-MS对21株标准沙门菌进行质谱数据采集,建立标准菌株数据库。相关菌株质谱数据通过菌株在相同培养环境不同培养时间下所得质谱数据进行优化。通过血清型鉴定方法筛选出200株野生沙门菌,并利用自建数据库和现有数据库对200株野生沙门菌进行MALDI-TOF-MS检测,对自建数据库进行评价。结果 Bruker原有数据库、自建数据库及合并数据库对200株野生沙门菌鉴定,阳性率分别为87.5%、97.0%、97.5%。21株标准沙门菌株数据库按亲缘关系远近可分为3大类。主成分分析发现不同菌种之间质谱数据有一定的差异,个别菌种在不同培养条件下培养,质谱数据变化不明显。结论本研究建立的标准菌库对野生沙门菌的鉴定准确性高,适用于实际检测。研究结果丰富了已有的数据库,同时也为其他细菌菌库的建立提供了很好的借鉴。
[Abstract]:Objective to use matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Methods 21 standard Salmonella strains were collected by mass spectrometry using MALDI-TOF-MS. The standard strain database was established. The mass spectrum data of related strains were optimized by mass spectrometry data obtained from the strains in the same culture environment and different culture time. 200 wild Salmonella strains were screened by serotype identification method. The self-built database and the existing database were used to detect 200 wild Salmonella strains by MALDI-TOF-MS, and the self-built database was evaluated. Results the original database of Bruker was used. The positive rates of 200 wild Salmonella strains were 87.5% and 97.0%, respectively. 97.5.21 standard Salmonella strains database can be divided into three categories according to the genetic relationship. Principal component analysis found that there are certain differences between different species of mass spectrum data. There was no obvious change in mass spectrum data of individual strains cultured under different culture conditions. Conclusion the standard bacterial bank established in this study has high accuracy for identification of wild Salmonella. The results enrich the existing database and provide a good reference for the establishment of other bacterial banks.
【作者单位】：中国人民武装警察部队后勤学院天津市职业与环境危害防制重点实验室;国家食品安全风险评估中心;
【基金】：国家自然科学基金(No.21077132) 武警后勤学院青年基金应用类项目(No.whk201302)
【分类号】：R378
【正文快照】： 沙门菌是肠杆菌科中一类常见的致病菌属,其致病因素复杂多变,是导致人类食物中毒的主要致病菌之一。近年来由其导致的食源性疾病事件持续增加,不但严重危害人类身体健康,而且给人类社会造成巨大经济损失[1-3]。沙门菌菌型繁多,传统的生化和血清学分型方法准确可靠,但操作复杂,

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