湖南省日本血吸虫DNA多态性的研究

发布时间：2018-01-11 10:22

本文关键词：湖南省日本血吸虫DNA多态性的研究　出处：《湖南农业大学》2006年硕士论文　论文类型：学位论文

【摘要】：对湖南省日本血吸虫DNA多态性进行研究，旨在阐明湖南省日本血吸虫种内的遗传变异，为进一步的分子遗传学和分子诊断学研究奠定基础。采用PCR扩增、克隆、测序和序列分析等方法对湖南省日本血吸虫核糖体DNA(rDNA)内转录间隔区(ITS)序列、线粒体(mtDNA)NADH脱氢酶亚单位Ⅳ基因(nad4)和细胞色素c氧化酶笫Ⅰ亚基(cox1)序列的多态性进行研究。结果表明：1．首次在国内报道湖南省长沙及岳阳钱粮湖农场日本血吸虫ITS序列，其中日本血吸虫ITS-1全序列的报道是国际上首次。与GenBanK中收录的日本血吸虫部分ITS-1及ITS-2全序列做比较，发现它们之间有一定差异。2．nad4基因序列的PCR扩增引物是作者根据GenBanK中收录的日本血吸虫与肝片吸虫的nad4基因序列设计的一对兼并引物，扩增片段为500bp左右。日本血吸虫nad4基因序列的报道在国内是首次。不同的代表性样品nad4序列差异明显，碱基差异在1.0％～2.9％之间。3．cox1基因序列分析表明，采自湖南岳阳君山、岳阳汨罗的样品cox1序列之间有一定差异，碱基差异在0.2％～1.2％之间。本研究证明了ITS、nad4及cox1三段序列确为理想的种内变异研究的遗传标记，研究结果有助于日本血吸虫的生活史、传播途径、流行病学、种群遗传学的研究和其相关疾病的诊断。
[Abstract]:Research on the Hunan province of Schistosoma japonicum DNA polymorphism, to elucidate the genetic variation of Schistosoma japonicum in Hunan Province, lay a foundation for study on molecular genetics and molecular diagnosis. Further study by PCR amplification, cloning, sequencing and sequence analysis and other methods of Hunan Province on Schistosoma japonicum ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence (mtDNA), mitochondrial NADH dehydrogenase subunit gene (nad4) and cytochrome c oxidase subunit I (cox1) in the study of the polymorphic sequences. The results showed that: 1. for the first time in China reported in Hunan province and Yueyang Changsha farm of Schistosoma japonicum ITS sequence, the whole sequence of Schistosoma japonicum ITS-1 report is the first international. With GenBanK included ITS-1 and Schistosoma japonicum ITS-2 sequence comparison, found.2.nad4 gene sequence differences between them is PCR primer A pair of primers designed according to the sequence of nad4 gene of Schistosoma japonicum and Fasciola GenBanK included in the amplified fragment was about 500bp. Schistosoma japonicum nad4 gene sequence reported in China for the first time. A representative sample of nad4 sequence differences obviously, alkali difference in 1% ~ 2.9% between.3.cox1 gene sequence analysis showed that collected from Hunan Yueyang Junshan, there are some differences between the samples of cox1 Series in Yueyang Miluo, base difference at 0.2% ~ 1.2%.
This study proves that ITS, nad4 and cox1 three segment sequences are ideal genetic markers for intraspecific variation research. The research results are helpful for Schistosoma japonicum's life history, transmission route, epidemiology, population genetics research and related diseases diagnosis.

【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R383

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