江苏宜兴地区丙型肝炎病毒基因型分布和包膜区变异研究

发布时间：2018-01-12 06:43

本文关键词：江苏宜兴地区丙型肝炎病毒基因型分布和包膜区变异研究　出处：《南京医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：目前全世界丙型肝炎病毒(HCV)的感染者已达到1.8亿，平均感染率约为3％。每年有300万到400万的新发病例。HCV是单股正链RNA病毒，HCV感染所致的丙型肝炎慢性化率高达75％～85％，可导致肝脏慢性炎症坏死和纤维化，部分患者可发展为肝硬化甚至肝细胞癌，对人类的健康和生命构成了严重威胁。因此，深入进行丙型肝炎的分子流行病学研究，探索与丙型肝炎慢性化相关的因素，以采取有效的预防控制措施已成为当务之急。本课题以江苏省宜兴市为研究现场，从病原学角度开展了以人群为基础的丙型肝炎分子流行病学研究。第一部分：江苏宜兴地区丙型肝炎病毒基因型分布研究 HCV基因组的核苷酸序列具有高度的变异性，目前主要分为六个基因型，即1～6型。不同基因型存在明显的地区分布差异，而且HCV的基因型对判断丙型肝炎治疗的难易程度及其预后具有重要的意义，故了解本地区HCV基因型分布特征作为进一步深入研究丙型肝炎慢性化的基础资料有其必要性。本研究采用Simmonds基因分型方法，依据HCV的保守区段5′非编码区(5′NCR)设计1、2、3和1b型特异性引物，逆转录-聚合酶链反应(RT-PCR)方法扩增分型，并对不同基因型在人群中的分布作统计描述。主要结果如下： 1．HCV RNA阳性检测和基因分型研究检测158份抗HCV阳性血清，其中95份HCV RNA阳性，阳性率为60.1％，基因分型结果1b型80例(84.2％)，2a型5例(5.3％)，，1b／2a型混合感染5例(5.3％)，分型不明确者5例(5.3％)。
[Abstract]:At present, the number of people infected with hepatitis C virus (HCV) in the world has reached 180 million, the average infection rate is about 30.There are 3 million to 4 million new cases every year. HCV is a single-stranded positive strand RNA virus. The chronic rate of hepatitis C caused by HCV infection is as high as 75% 85%, which can lead to chronic inflammation, necrosis and fibrosis of liver, and some patients can develop into liver cirrhosis or even hepatocellular carcinoma. It poses a serious threat to human health and life. Therefore, the molecular epidemiology of hepatitis C is deeply studied, and the factors related to the chronic hepatitis C are explored. It has become an urgent task to take effective preventive and control measures. In this paper, a population-based molecular epidemiology study of hepatitis C was carried out in Yixing City, Jiangsu Province, from the point of view of etiology. Part I: distribution of hepatitis C virus genotypes in Yixing area, Jiangsu Province The nucleotide sequence of HCV genome is highly variable, which is mainly divided into six genotypes, I. e., genotype 1: 6. There are obvious regional differences among different genotypes. Moreover, the genotype of HCV plays an important role in judging the degree of difficulty in the treatment of hepatitis C and its prognosis. Therefore, it is necessary to understand the distribution of HCV genotypes in this area as the basic data for further study of chronic hepatitis C. Simmonds genotyping method is used in this study. According to the conserved 5'non-coding region of HCV, the specific primers of 1k2m3 and 1b were designed and amplified by reverse transcription-polymerase chain reaction (RT-PCR). The distribution of different genotypes in the population was statistically described. The main results were as follows: 1. Detection and genotyping of HCV RNA The positive rate of HCV RNA was 60.1%, and the genotyping result was 1b in 80 cases (84.2%). There were 5 cases of type 2a mixed infection of 1 b / 2a and 5 cases of mixed infection of type 2 a, 5 cases were divided into 5 cases and 5 cases were divided into 5 cases and 5.3% respectively.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

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