PolyI：C持续刺激对小鼠BMDC成熟的影响和CpG持续刺激对小鼠BMDC作用的基因芯片分析

发布时间：2018-01-12 07:34

本文关键词：PolyI：C持续刺激对小鼠BMDC成熟的影响和CpG持续刺激对小鼠BMDC作用的基因芯片分析　出处：《第二军医大学》2006年硕士论文　论文类型：学位论文

更多相关文章： 树突状细胞 Polyl:C CpG 基因芯片

【摘要】：目的:研究PolyI:C和CpG持续刺激对小鼠骨髓树突状细胞(DC)成熟的影响。方法:BMDC分为PolyI:C或CpG持续刺激组、短期刺激组和对照组;流式检测表型和摄取抗原的能力,ELISA检测细胞因子水平,混淋实验检测提呈抗原的能力。基因芯片方法分析基因表达谱变化。结果:PolyI:C早期持续刺激可抑制BMDC的成熟,表现为:形态上BMDC集落小、毛刺少;表达Iab、CD80、CD86和CD40水平低;分泌IL-6、IL-12p40、IL-1β、TNF-α、IFNγ和MMP9水平较低,而IL-10、TGFβ较高;摄取抗原的功能维持在高水平,而刺激同种异基因T细胞增殖能力不足。基因芯片结果显示:CpG持续刺激组BMDC中许多DC常见活化信号基因均比CpG短期刺激组BMDC表达下调2倍以上;进一步说明CpG持续刺激可抑制BMDC的成熟。初步体内实验结果提示:CpG持续作用的BMDC对小鼠特异性抗体产生有一定的抑制作用。结论:PolyI:C或CpG持续刺激可抑制DC的发育成熟,可能是持续严重感染时免疫功能低下的原因。
[Abstract]:Objective: to study the effect of PolyI:C and CpG continuous stimulation on the maturation of bone marrow dendritic cells (DC) in mice. Short-term stimulation group and control group; The ability of flow cytometry to detect phenotypes and uptake of antigens and to detect cytokines by Elisa. The ability of presenting antigen was detected by mixed elution assay. The gene expression profile was analyzed by gene chip method. Results the mature of BMDC could be inhibited by early sustained stimulation of 1: PolyI: C. The results were as follows: the BMDC colony was small and the burr was less in morphology; The expression levels of CD80, CD86 and CD40 were low. The levels of TNF- 伪 IFN- 纬 and MMP9 were lower and IL-10 TGF- 尾 were higher. The function of antigen uptake was maintained at a high level. But the ability of stimulating allogeneic T cell proliferation was not enough. The results of gene chip showed that:. The expression of many DC activation signal genes in BMDC of CpG continuous stimulation group was more than 2 times lower than that of CpG short-term stimulation group. The results showed that the sustained stimulation of CpG could inhibit the maturation of BMDC. The preliminary in vivo experiment indicated that the BMDC with the sustained action of CpG could inhibit the production of specific antibody in mice. Continuous stimulation of PolyI:C or CpG could inhibit the development and maturation of DC. This may be the cause of low immune function during persistent severe infection.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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