小鼠新分子mPEBP4的克

发布时间：2018-01-14 09:25

本文关键词：小鼠新分子mPEBP4的克隆、鉴定及其功能研究　出处：《浙江大学》2006年硕士论文　论文类型：学位论文

【摘要】：磷脂酰乙醇胺结合蛋白(Phosphatidylethanolamine-binding protein,PEBP)家族是一个分子量在21-23kDa之间的碱性蛋白家族。最早是因为能与细胞膜成份磷脂酰乙醇胺(Phosphatidylethanolamine,PE)结合而在牛脑中被发现。PEBP是一类进化上非常保守的蛋白家族,广泛存在于植物、疟原虫、线虫、果蝇及哺乳动物(包括牛、猴、人等)中。PEBP家族成员具有多种生物学功能,如结合脂质、抑制丝氨酸蛋白酶活性等。有些PEBP成员还能作为十一氨基酸多肽海马趾类胆碱神经刺激肽(HCNP)前体蛋白,在海马的发育中起着重要的作用。Raf-1和MEK都能与PEBP中高度保守的区域结合,而hPEBP1(又称为Raf激酶抑制蛋白,RKIP,Raf kinase inhibitory protein)能抑制Raf-1激酶和丝裂原激活的蛋白激酶的活性,使MEK和Raf-1复合物解离,从而抑制了MEK的活化。最近有研究还表明PEBP的一家族成员RKIP的表达还能抑制前列腺癌的转移。在我们以往从人骨髓基质细胞(BMSC)cDNA文库中自主发现的一种新型PEBP家族成员即人磷脂酰乙醇胺结合蛋白4(human phosphatidylethanolamine(PE)-bindign protein 4,hPEBP4)并证明其在乳腺癌高表达和发挥抗凋亡作用的基础上,本研究拟克隆hPEBP4的小鼠同源分子mPEBP4,并对其生物学功能进行初步研究。第一部分、mPEBP4的克隆和序列分析、组织细胞分布和细胞定位研究如上所述,PEBP是一类能与磷脂酰乙醇胺(PE)结合的蛋白,该家族的所有成员都含有与PE结合的保守区域(PBP)。人磷脂酰乙醇胺结合蛋白4(human phosphatidylethanolamine(PE)-binding protein 4,hPEBP4)是我们从人骨髓基质细胞(BMSC)cDNA文库中自主发现的一种新型PEBP家族成员,其全长cDNA为874bp,编码227氨基酸的蛋白。我们以往的研究表明该蛋白在核酸和氨基酸水
[Abstract]:Phosphatidylethanolamine binding protein (Phosphatidylethanolamine-binding protein PEBP) is a family of molecular weight between 21-23kDa alkaline protein family. The earliest is due to cell membrane component and phosphatidylethanolamine (Phosphatidylethanolamine, PE) and was found in the bovine brain.PEBP is an evolutionarily conserved protein family. Widely exists in plants, Plasmodium, nematode, Drosophila and mammals (including cows, monkeys, etc.) in the.PEBP family members with a variety of biological functions, such as lipid binding, inhibition of serine protease activity. Some members of PEBP can be used as a eleven amino acid polypeptide of hippocampal cholinergic neurostimulating peptide (HCNP) precursor protein in the hippocampus. The development plays a role of.Raf-1 and MEK are important conserved region and PEBP binding, and hPEBP1 (also called Raf kinase inhibitor protein, RKIP Raf, kinase inhibitor Y protein) can inhibit Raf-1 kinase and mitogen activated protein kinase activity, the MEK and Raf-1 complex dissociation, thereby inhibiting the activation of MEK. Recent studies also showed that the expression of PEBP can transfer a member of the family of RKIP inhibits prostate cancer. In our previous from human bone marrow stromal cells (BMSC) a a new member of the PEBP family of self discovery in cDNA Library of human phosphatidylethanolamine binding protein 4 (human phosphatidylethanolamine (PE) -bindign protein 4, hPEBP4) and prove its high expression in breast cancer and play the basic role of anti apoptotic, we cloned hPEBP4 mouse homologous molecule mPEBP4, and preliminary study on the biological function.
The first part, cloning and sequence analysis of mPEBP4, tissue cell distribution and cell localization
As mentioned above, PEBP is a kind of phosphatidylethanolamine binding protein (PE), all members of the family contain the conserved region combined with PE (PBP). Human phosphatidylethanolamine binding protein 4 (human phosphatidylethanolamine (PE) -binding protein 4, hPEBP4) is from human bone marrow stromal cell (BMSC) is a new member of the PEBP family are found in the cDNA library. The full-length cDNA is 874bp, encoding 227 amino acid protein. Our previous studies showed that the protein nucleic acid and amino acid in water

【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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