胆汁淤积性肝硬化大鼠模型的改良

发布时间：2018-01-14 23:25

本文关键词：胆汁淤积性肝硬化大鼠模型的改良　出处：《中国比较医学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的构建肝纤维化大鼠模型,并对经典结扎胆总管(BDL)复制模型方法进行适当改进。方法 80只SD雄性成熟大鼠,按随机数字表法分为A组40只、B组40只,分别运用胆总管结扎法和肝门部肝总管缝扎法先后两次造模,各取其中10只为假手术组进行对照,术后1周眼眶取血采用酶联免疫吸附法检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)、总胆红素(TBIL)、直接胆红素(DBIL)、γ-谷氨酰转肽酶(GGT)、白蛋白/球蛋白(A/G),术后4周HE染色观察大鼠肝组织病理学变化,采用免疫组化染色分析肝脏组织α-SMA和CK-19表达水平。结果两种方法均表现出明显肝功能损害;标本胆小管增生明显,肝脏假小叶形成,达到早期肝硬化,肝脏α-SMA和CK-19表达水平明显升高,胆总管结扎组死亡率为66.7%,肝门部肝总管缝扎组死亡率为26.7%。结论肝门缝扎法可成功建立胆汁淤积性肝硬化大鼠模型,能够明显降低模型动物死亡率,提高模型质量及实验效率。
[Abstract]:Objective to establish a rat model of hepatic fibrosis and to improve the method of classical ligation of common bile duct (BDL). Methods 80 adult SD male rats were randomly divided into group A (n = 40) and group A (n = 40). In group B, 40 rats were made models by common bile duct ligation and hepatic hilar common bile duct suture respectively, 10 of them were used as control group. 1 week after operation, the serum levels of aspartate, alanine aminotransferase (alt), alkaline phosphatase (ALP) and total bilirubin (TBIL) were detected by enzyme-linked immunosorbent assay (Elisa). The liver histopathological changes of rats were observed by HE staining 4 weeks after operation, including direct bilirubin bilirubin dbl, 纬 -glutamyl transpeptidase (GGT), albumin / globulin A / G (P < 0.05). The expression levels of 伪 -SMA and CK-19 in liver tissue were analyzed by immunohistochemical staining. The expression of 伪 -SMA and CK-19 in the liver was significantly increased. The death rate of the common bile duct ligation group was 66.7%. Conclusion the hepatic portal ligation method can successfully establish the rat model of cholestatic cirrhosis, which can obviously reduce the mortality of the model and improve the quality and experimental efficiency of the model.
【作者单位】：南京中医药大学;解放军81医院;江苏省中医药研究院;南京中医药大学附属中西医结合医院;中国中医科学院江苏分院;
【基金】：国家自然科学基金(No.81060255) “江苏省六大人才”高峰项目(2013-WS-048) 江苏省科技厅技术基础设施建设计划项目(BM2008152)
【分类号】：R-332
【正文快照】： 肝纤维化是多种慢性肝病发展为肝硬化的必经阶段,阻断肝纤维化是慢性肝病治疗中的关键问题[1]。为了更好地研究肝纤维化的发生机制以及药物对该病的疗效和治疗途径,需要建立稳定、高效、可靠的肝纤维化动物模型[2,3]。国内文献对胆总管结扎模型建立的手术操作过程描述过于简单

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