女性尿道括约肌的神经分布：胎儿免疫组化研究

发布时间：2018-01-15 01:27

本文关键词：女性尿道括约肌的神经分布：胎儿免疫组化研究　出处：《青岛大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的支配女性尿道括约肌神经纤维精确的定性、定量和定位结论还没有清楚地建立。以尸体解剖为基础的有关阴部神经和自主神经纤维的大量解剖学研究为我们提供了许多矛盾的结论。本实验设计在于通过对女性胎儿尿道的免疫组化分析，识别女性尿道括约肌的神经分布。方法选取12例21周至40周的正常女性胎儿尿道，分别进行石蜡包埋、胆碱乙酰基转移酶(choline acetylcatransferase，CHAT)及神经肽Y(neuropeptide Y，NPY)免疫组织化学染色(SP法)，，并对其结果进行半定量和定量统计学分析。结果从尿道内口至外口，神经肽Y阳性区域显色范围逐渐变小、强度减弱，近1／3与远1／3，中1／3与远1／3之间差异均有极显著性(P(0.01)，但近1／3与中1／3之间差异无显著性(P＞0.05)。胆碱乙酰基转移酶阳性区域主要位于尿道中1／3，近1／3次之，远1／3最弱，远、中、近端三者差异均具有极显著性(P＜0.01)。结论1．胆碱乙酰基转移酶及神经肽Y阳性区域主要位于女性尿道的中1／3，统计学上具有显著性差异，是胆碱能及肾上腺素能神经递质集中的部位；2．横纹括约肌开始于尿道近1／3的远端，位于平滑肌的外侧，向远端逐渐增厚，尿道中1／3为高峰，以后逐渐变薄；3．在女性胎儿尿道的中1／3处，平滑肌与横纹括约肌之间没有明显的界限，可清楚地看到平滑肌和横纹肌纤维互相交错分布，逐渐过渡，形成所谓的尿道括约肌复合体。尿道括约肌的胆碱乙酰基转移酶及神经肽Y免疫组织化学染色对理解参与控尿的尿道括约肌神经支配提供了很有价值的帮助，对临床实践具有指导意义。
[Abstract]:Objective to control female urethral sphincter nerve fiber qualitative accurate, quantitative and positioning conclusion is not clearly established. A study on anatomy of the autopsy on the pudendal nerve and autonomic nerve fibers as the foundation provided conflicting results for us. The experimental design is through the immune group of female fetuses urethral nerve distribution analysis identification of female urethral sphincter.
The normal fetal female urethral methods 12 cases 21 to 40 weeks, respectively, were embedded in paraffin, choline acetyltransferase (choline acetylcatransferase, CHAT) and neuropeptide Y (neuropeptide Y NPY) immunohistochemical staining (SP method), and the results were analyzed quantitatively and semiquantitatively statistically.
The results from urethral mouth to mouth, neuropeptide Y positive area color range becomes smaller, the intensity of nearly 1 / 3 and 1 / 3 in the far, 1 / 3 and 1 / 3 far between the differences were very significant (P (0.01), but nearly 1 / 3 and 1 / 3 no significant difference (P > 0.05). B choline acyltransferase positive areas were mainly located in the urethra in 1 / 3, nearly 1 / 3, 1 / 3 is the weakest, far in the proximal three significantly (P < 0.01).
Conclusion 1. choline acetyltransferase and neuropeptide Y positive areas were mainly located in the female urethra in 1 / 3, a significant difference is the cholinergic and adrenergic neurotransmitter concentration areas; 2. in the distal urethral sphincter started nearly 1 / 3, is located outside the smooth muscle, gradually thickened distal to the in 1, urethral / 3 peak, then gradually thinning; 3. in the female fetus urethral / 1 3, there was no obvious difference between the smooth and striated sphincter, can clearly see the striated and smooth muscle fibres interveined each other, gradually, the formation of urethral sphincter complex matter.
Immunohistochemical staining of choline acetyltransferase and neuropeptide Y in urethral sphincter can provide valuable help for understanding the innervation of urethral sphincter, and is of guiding significance for clinical practice.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R323

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