日本血吸虫多价DNA疫苗的研究

发布时间：2018-01-15 02:08

本文关键词：日本血吸虫多价DNA疫苗的研究　出处：《江苏省血吸虫病防治研究所》2005年硕士论文　论文类型：学位论文

【摘要】：日本血吸虫病至今仍是一种严重危害公众健康的公共卫生问题。我国每年要投入大量的人力、物力防治血吸虫病,但由于血吸虫保虫宿主多,钉螺分布广且难以消灭,吡喹酮化疗为主的血防策略难以阻断传播,再感染频繁发生,血吸虫病的流行状况仍没有得到根本改善。研制安全、有效的血吸虫病疫苗,控制家畜感染,减少传播来源和对人体的威胁是WHO 提出的综合防治血吸虫病中的一个主要措施,且放在优先发展的位置上。目前已有6 种曼氏血吸虫候选疫苗分子得到WHO/TDR 认可。DNA 疫苗作为一种划时代的新技术,在抗血吸虫感染领域里也取得了很多进展。本实验室已构建的日本血吸虫磷酸丙糖异构酶(TPI)DNA 疫苗,免疫小鼠获得部分的保护作用,且在保虫宿主猪研究中发现除具有抗感染作用外,还能减轻宿主肝脏虫卵肉芽肿炎症反应,肉芽肿平均面积明显减小;管晓虹等对日本血吸虫单克隆抗独特型抗体NP30 的研究发现,NP30 分子具有较好的抗感染免疫和抗病免疫功效,其H链CDR3区可能代表了肠相关抗原(GAA)的抗原分子模拟,起到相似的主动免疫作用,人工设计的日本血吸虫单克隆抗独特型抗体NP30 CDR3 区6 倍重复表位基因(CDR3)6的表达产物保留了抗体的亲和性和特异性;因此TPI 和(CDR3)6 均为日本血吸虫疫苗的候选分子,且两者的作用形式和机理似各不相同,分别为酶性抗原分子与抗原模拟表位基因。本研究构建含TPI 和(CDR3)6 这两种抗原分子的多价DNA 疫苗,观察其免疫保护效果,并初步探讨其作用机理。鉴于本实验室对于免疫刺激序列的研究发
[Abstract]:Schistosomiasis is still a serious public health problem in public health. China has to spend a lot of manpower and material resources of schistosomiasis prevention, but due to Schistosoma infection, snail widely distributed and difficult to destroy, schistosomiasis control strategy praziquantel to prevent the spread of infection, and frequent, the schistosomiasis epidemic situation is still have not been fundamentally improved. The development of safe and effective vaccine for schistosomiasis control, livestock infection, reduce the spread of source and threat to human is one of the main measures of comprehensive prevention and control of schistosomiasis proposed by WHO, and placed in the priority position. There are 6 kinds of vaccine candidate molecules of Schistosoma mansoni WHO/TDR recognized as a.DNA vaccine a new technology of epoch-making, much progress has also been made in the field of anti schistosome infection. The laboratory has constructed the Japanese blood fluke Triose phosphate isomerase (TPI) DNA vaccine immunized mice protection effect, and the infection of pigs found besides anti infection effect, but also reduce the host liver granuloma inflammatory reaction, the average area of granuloma obviously decreased; Study on Schistosoma japonicum monoclonal anti idiotypic antibody NP30 Guan Xiaohong found. The NP30 molecule has good anti infection immunity and disease resistance of immune function, the H chain CDR3 region may represent the gut associated antigen (GAA) to simulate antigen molecules, plays a role similar to active immunization, artificial Schistosoma japonicum monoclonal anti idiotypic antibody NP30 CDR3 region 6 times repeated epitope gene (CDR3) expression product 6 retained the affinity and specificity of antibodies; therefore TPI and (CDR3) of all 6 candidate molecules of Schistosoma japonicum vaccine, and their mechanism of action form and may vary, respectively. In order to mimic epitope genes for enzyme antigen molecules and antigens, we constructed a multivalent DNA vaccine containing two antigens of TPI and (CDR3) 6. We observed its protective effect and explored its mechanism.

【学位授予单位】：江苏省血吸虫病防治研究所
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

【引证文献】